The Crosslink Reporter Peaks page lists all spectra that contain the special diagnostic pair of ions that originate from the cleaved crosslinker. The application searches for all spectra containing a potential diagnostic ion pair for crosslinked peptides.
The following table describes the columns on the Crosslink Reporter Peaks page of the result file.
Column | Description |
|---|---|
Charge | Displays the charge of the peptide. |
Charge xA | Displays the charge of the reporter isotopes associated with the first peptide. |
Charge xB | Displays the charge of the reporter isotopes associated with the second peptide. |
Crosslink Strategy | Displays the acquisition mode. |
Crosslinker | Displays the name of the crosslinking reagent. |
Diagnostic Peaks P-Value | Displays the score calculated for the detected reporter isotopes. |
Fragmentation Scan Numbers | Displays all scan numbers that are expected to contain the proper fragmentation events needed to identify the crosslinker. |
Fragmentation Spectrum IDs | Displays all unique spectrum identifications that are expected to contain the proper fragmentation events needed to identify the crosslinker. |
Intensity | Displays the intensity of the precursor ion. |
M/z [Th] | Displays the mass-to-charge ratio of the precursor isotope. |
M/z xA [Th] | Displays the mass-to-charge ratio of the reporter isotopes associated with the first peptide. |
M/z xB [Th] | Displays the mass-to-charge ratio of the reporter isotopes associated with the second peptide. |
Mass [Da] | Displays the calculated monoisotopic mass of the precursor isotope. |
Pre Scan Number | Displays the scan number of the prescan (MS scan) in the raw data file, which is expected to produce the reporter ions. |
Pre Spectrum ID | Displays the unique spectrum identification of the prescan (MS scan), which is expected to produce the reporter ions. |
Retention Time [min] | Displays the retention time when the crosslinker peptide was observed. |
Scan Number | Displays the scan number of the scan in the raw data file. |
Spectrum File Path | Displays the path and name of the raw data file. |
Spectrum ID | Displays the unique spectrum identification. |
Unmodified Mass xA [Da] | Displays the unmodified mass of the first peptide. |
Unmodified Mass xB [Da] | Displays the unmodified mass of the second peptide. |