When

Related messages that require a flow cell check appear in the Audit Trail.

Follow these steps

  1. Check that the flow cell is installed properly.

  2. Remove and re-install the flow cell.

  3. Flush the flow cell with pure HPLC-grade water with a flow rate of >1 mL/min for several minutes (Nano flow cells: Flush with pure HPLC-grade water with the maximum possible flow rate for the respective range for several minutes).

  4. Install a different flow cell and repeat the action during which the message appeared with the flow cell that was previously installed.

Result

Steps

If the message disappears with a different flow cell

The problems are caused by the flow cell that was previously installed or the flow path or components in the flow path before the detector.

If the message still appears

The problem is caused by the flow path or components in the flow path before the detector or by the detector.

Install a diagnostic cell and repeat the action during which the message appeared with the flow cell that was previously installed.

  • If the message disappears with the diagnostic cell: The problems are caused by the flow path or components in the flow path before the detector.

  • If the message still appears: The problems are caused by the detector.