As a standard, when setting the data collection rate, Chromeleon automatically sets the optimum response time and peak width. As an advanced option, response time and peak width can be set individually.

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Disabling the link between the data collection parameters and setting these parameters individually may lead to increased noise or increased peak widths.

Observe the following guidelines when changing the response time and peak width proposed by Chromeleon:

  • Ensure that response time and peak width match the setting for the data collection rate.

  • The response time should be about 30% of the peak width at half-height of the narrowest peak of interest.

    A longer response time allows more averaging of the signal and results in less short-term noise. However, if the selected response time is too long, this can result in reduced peak heights and asymmetrical peak shapes. If a separation of peaks is done that follow closely to each other, the long response time can result in bad peak separation. When set correctly, the response time significantly reduces baseline noise, and reduces peak height only slightly.

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For best possible combinations of data collection rate, response time and peak width, enable the Link data collection parameters check box in the detector settings of the Chromeleon Instrument Method Wizard or Instrument Method Editor.