Single-channel mode is the default mode of operation for the detector. As this mode provides the highest possible data rates and best signal-to-noise ratio, always use the single-channel mode for routine operation of the detector.
Consider the following for operating the detector in single-channel mode:
Sample components should absorb strongly at the selected wavelength. Therefore, select a wavelength on an absorption maximum.
At the selected wavelength no other sample components should absorb that can falsify the peaks of the sample components of interest.
If the absorbance spectra of the sample components differ significantly, the measurement wavelength can be switched any time and as often as required during an acquisition.
The switching is considerably shorter than one second and should be performed when no sample components of interest are present in the flow cell.
While the wavelength is switched, no absorption measurement takes place for a short period of time. If the wavelength is switched during an analysis of a sample component, the peak area changes, and a correct quantification is not possible. You can choose between three different baseline behaviors, when switching the wavelength (for details, see Baseline Behavior).