When setting up flow connections, follow these rules and recommendations:

Flow connections can be filled with hazardous substances. Observe the warning messages and precautionary statements presented in Safety Precautions.

NOTICE

Particulate matter from other system modules and components can deposit in the flow cell and clog it.

  • Before you connect the flow cell to the flow path, make sure that you thoroughly flush the modules in the system flow path upstream of the device to waste.
  • When you install devices or components to the system, always flush them to waste before connecting them in the system flow path. To flush the Vanquish modules, follow the instructions in the Vanquish System Operating Manual.

NOTICE

Flow cells are highly sensitive to contamination, clogging and high backpressures. Even if the pressure exceeds the upper limit for a very short time only, the flow cell may be permanently damaged. Observe the following notes when connecting the flow cell to the system flow path:

  • When connecting a component in the flow path after the flow cell, observe the specified backpressure for the flow cell.
  • Use only clean Viper capillaries which were provided for the flow cell and which have been properly protected by their cap before.
  • Use only the waste line which was provided for the flow cell.
  • Avoid clogging of the flow cell or waste line.
  • Improperly set up flow connections can lead to leaks on the flow cell or even destroy the flow cell. Do not invert the flow cell inlet and outlet.
  • If you install switching valves or fraction collectors downstream of the flow cell, operating those under flow may result in pressure spikes that can destroy the flow cell. Ensure that pressure spikes do not exceed the flow cell pressure specification.

Follow these steps

To set up the flow connections and complete the installation of the device, follow these steps:

  1. Set up the flow connections to the flow cell (see Flow Connections to the Flow Cell).

  2. Connect the device to the drain system (refer to the Vanquish System Operating Manual).

For installation instructions, guidelines, and handling recommendations, see Connecting Fittings, Capillaries, and Tubing.