Parts required

  • Mixing system, volume: 100 µL, comprising a static mixer (volume: 75 µL) and a capillary mixer (volume: 25 µL)

  • Capillary to connect the pump to the autosampler

Tools and additional items required

  • Depending on the fitting connection on the capillaries

    • Viper capillary: Viper knurl (usually present on the capillary)

    • Conventional capillary (non-Viper): Open-end wrench, size 1/4"

  • Solvent suitable for purging

Preparations

  1. If not yet done: Remove the mixing system with which the pump is shipped (see Removing the Mixing System).

  1. For easier installation, remove the seal wash detector assembly (funnel and detector). Grasp and pull the assembly upward by the funnel. The detector including the tubing and cable can remain connected to the funnel.

Removing the seal wash detector assembly
Figure: Removing the seal wash detector assembly

No.

Description

1

Funnel, seal wash detector

2

Seal wash detector

3

Detector cable

4

Seal wash line

Follow these steps

  1. Connect the capillary mixer on the purge/sensor unit. Mind the direction of flow through the capillary mixer (indicated by the arrow on the mixer).

  2. TIP

    To facilitate installation of the static mixer, loosely tighten the capillary mixer on the purge/sensor unit so that you can easily access the free end of the capillary.

  3. Connect the static mixer to the capillary mixer. Mind the direction of flow through the static mixer (indicated by the arrow on the mixer).

  4. Turn and insert the assembly of static mixer and capillary mixer into the pump so that the knurl of the capillary mixer rests on the edge of the purge/sensor unit.

100 µL mixing system installed
Figure: 100 µL mixing system installed

No.

Description

1

Static mixer

2

Capillary mixer

3

Knurl resting on the purge/sensor unit

  1. Tighten the capillary mixer on the purge/sensor unit.

  2. Reinstall the seal wash detector assembly in the pump (see Replacing the Seal Wash Detector).

  3. Connect the static mixer outlet to the autosampler injection valve (see Connecting the Pump and Autosampler).

  4. In the Chromeleon software, set the StaticMixer property to the value indicated on the static mixer. If the property is not set to the correct value, the leak tests may not provide reliable results.

  5. With Chromeleon 7.2.8 and later: In the Chromeleon software, set the CapillaryMixer property to the value indicated on the capillary mixer.

  6. Apply the flow rate of your application and have the pump deliver for a short time.

  7. Inspect the flow connections on the mixer for indications of leakage before resuming operation.

  8. In the Chromeleon software, consider updating the Predictive Performance information for the static mixer (MixerChanged command).

When operating the pump, observe the following

  • Operating the pump with a mixing system with a volume lower than the volume installed in the pump upon shipment reduces the gradient delay volume but increases the ripple.

  • The maintenance procedures are the same as for the mixing system with which the pump was shipped (see Mixing System).