To interrupt operation for a longer period, follow the instructions in this section.

TIP

Shutting down the device affects the operation of the system. When shutting down the device, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).

  1. Remove the column.

  2. Flush the system with an appropriate solvent (minimum HPLC-grade). Observe the following:

TIP

With a Vanquish Core system that has been modified for using normal-phase compatible solvents and additives, refer to the information about the flushing liquid in the Considerations with Normal-Phase Compatible Solvents and Additives section in the Vanquish System Operating Manual.

Situation after Shutdown

If no additive is used

If an additive is used

Device remains in the laboratory after shutdown

Flush the system, for example with methanol. 100% acetonitrile should not be used.

Flush the system with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent.

Device shall be transported or shipped after shutdown

Flush the system with isopropanol.

Flush the system first with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent. Afterward, flush the system with isopropanol.

  1. Turn off the pump flow and wait until the system pressure is down to zero.

  2. Disconnect the solvent lines. See Disconnecting the Solvent Lines.

TIP

Removing the solvent lines from the tubing guides is usually not required (not even for replacing the slide-in module). However, when you remove the solvent lines from the tubing guides in the pump and all modules above the pump in the system stack, be careful not to pull on other tubing in the guides.

  1. Empty the seal wash system. See Emptying the Seal Wash System.

  1. The step depends as follows:

Situation

Steps

Device and all other system modules remain in the system stack and are to be turned off

Turn off the system with the system power button on the system base.

Device shall be transported or shipped after shutdown

If one of the modules shall be removed from the system stack, turn off all system modules with their main power switch. Pressing the system power button will not be sufficient to turn off the power to the devices completely.

Follow the instructions in Transporting or Shipping the Device.

Disconnecting the Solvent Lines

  1. Unscrew the caps of the solvent reservoirs.

  2. Together with the caps, remove the solvent lines from the reservoirs.

  3. Protect the solvent line filters from contamination as appropriate.

  4. Empty the solvent lines. See Emptying the Solvent Lines.

  5. Disconnect the solvent lines on the pump inlets.

  6. Close the pump inlets with appropriate plugs.

Emptying the Seal Wash System

  1. Unscrew the cap of the seal wash reservoir.

  2. Together with the cap, remove the seal wash line from the seal wash reservoir.

  3. Remove the peristaltic tubing from the seal wash pump. When the tubing is removed, any liquid that may be present in the seal wash line, flows off to waste.