When
The pump is operated for the first time
To flush out the isopropanol that is present in some components of the pump when the pump is shipped from the manufacturing site
One or more solvent lines have been empty
TIP
To remove air bubbles or to change to a different type of solvent, follow the steps in Purging for Removing Air Bubbles or Changing to Different Solvent Type.
Items required
Solvents suitable for purging
To flush out the isopropanol when operating the pump for the first time, use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent.
Silicone tubing
Tissue
Syringe
TIP
For first time-operation: Silicone tubing and a syringe are available in the ship kit.
Preparations
To be able to generate negative pressure, connect an autosampler or another type of flow restriction to the pump outlet. If necessary, close the pump outlet with a fitting plug.
On the purge/sensor unit, attach silicone tubing to the purge outlet (if it is not yet connected).
Insert the syringe tip into the open end of the tubing.
Open the purge valve by turning the valve knob counterclockwise.
Follow these steps
In the Chromeleon software, set the channel that you want to purge to 100%.
Optional: In the Chromeleon software, adapt the settings for the purge flow or purge time if required.
Binary pump and dual pump: For purging from the keypad, verify that you have selected the pump head (pump) for which you want to perform the procedure.
Start the purge cycle. You can start the purge cycle from the Chromeleon software or by pressing the PURGE button on the keypad.
Use the syringe to draw enough solvent for filling the solvent line to the purge outlet.
When the liquid enters the silicone tubing, examine the solvent line. If the solvent line is completely filled with liquid and no air bubbles are visible, stop the purge cycle (from the Chromeleon software or by pressing the PURGE button).
Remove the syringe from the silicone tubing, absorbing any liquid that leaves the tubing with a tissue.
Route the free end of the tubing to the drain port outlet (see Guiding Liquids to Waste).
Perform the above steps for all channels, including those that are not used for the application.
If necessary, empty the syringe before starting the procedure for the next channel.
When purging is complete, close the purge valve by turning the valve knob clockwise.
NOTICE
Turn the valve knob only with your fingers. If the knob leaks, tighten a little more. Be careful; overtightening may damage the purge valve seals.
Empty the silicone tubing.
Dual pump only: If applicable, perform the purge procedure also for the other pump.
Emptying the Silicone Tubing
NOTICE
Always empty the silicone tubing when purging is complete. When the purge valve is closed, purge liquid remains in the tubing connected to the purge outlet. Leaving the liquid in the tubing for a longer period limits the tubing lifetime.
To absorb any liquid that might be leaving the purge outlet when the tubing is removed, place the tissue under the outlet.
On the purge/sensor unit, remove the silicone tubing from the purge outlet. The other end of the tubing remains in the purge drain port outlet.
Hold the tubing so that any liquid that is present in the tubing flows off through the drain port outlet.
On the purge/sensor unit, consider reconnecting the silicone tubing.