When
Damage or blockage of the seal wash lines
Peristaltic tubing: Every 6 months as preventive maintenance for the seal wash pump
Other seal wash lines: Once a year
Parts and additional items required
Seal wash lines as required:
Seal wash lines that connect the seal wash reservoir to the peristaltic tubing in the pump
For more information, refer to the Operating Manual for the Vanquish autosampler.
Seal wash lines that connect the components in the pump
Peristaltic tubing (PharMed tubing)
Fresh seal wash liquid
For an overview of the seal wash system, see the picture in Setting Up the Seal Wash System.
Tools required
Tubing cutter (optional)
Preparations
Unscrew the cap of the seal wash reservoir and remove the seal wash line together with the cap from the reservoir.
To empty the seal wash line, press down the lever of the seal wash pump. Release the lever when the line is empty.
Follow these steps
NOTICE
When you must remove a seal wash line from the tubing guides, be careful not to pull on other tubing in the guides.
Follow the sequence of steps for the seal wash line that you want to replace:
For the seal wash lines that connect the seal wash reservoir to the peristaltic tubing in the pump, follow the steps in the Operating Manual for the Vanquish autosampler.
For the peristaltic tubing, see Replacing the Peristaltic Tubing.
For the seal wash lines from peristaltic tubing to the pump heads and on to the seal wash detector, see Replacing the Seal Wash Lines between the Peristaltic Tubing and the Seal Wash Detector.
Replacing the Peristaltic Tubing
No. | Description |
---|---|
1 | Pump lever |
2 | Pump rotor |
3 | Tubing holder (peristaltic tubing) |
4 | Tubing connector |
5 | Seal wash line from autosampler (silicone tubing) |
6 | Peristaltic tubing |
7 | Seal wash tubing to pump head (silicone tubing) |
Disconnect the peristaltic tubing from the tubing connectors that connect it to the silicone tubing.
Press down the lever on the seal wash pump and remove the peristaltic tubing from the pump and from the tubing holders.
Connect the new peristaltic tubing to the silicone tubing using the tubing connectors. Cut tubing to required length, if applicable.
Insert the peristaltic tubing into the right tubing holder. Make sure that the tubing connector rests on the tubing holder. Push the tubing connector vertically down onto the holder if required.
Press down the lever on the seal wash pump and hold it in that position with one hand.
With your other hand, insert the tubing between the lever and the rotor and wind it around the rotor.
Release the lever.
Insert the peristaltic tubing into the left tubing holder.
Check that the peristaltic tubing is inserted properly in the tubing holders. If it is not, push the tubing into the holders. Make sure that the tubing is not pinched or squeezed in the holders.
Restarting the Seal Wash System
Retighten the reservoir cap hand-tight. The retaining guide must remain in the hole in the reservoir cap. If it does not, press the retaining guide into the hole so that the tubing is kept in place in the cap.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Flush the seal wash system, using fresh seal wash liquid (see Flushing the Seal Wash System).
Replacing the Seal Wash Lines between the Peristaltic Tubing and the Seal Wash Detector
Disconnect the seal wash tubing from the tubing connectors. Do not remove the tubing connectors.
Connect the new seal wash lines.
Retighten the reservoir cap hand-tight. The retaining guide must remain in the hole in the reservoir cap. If it does not, press the retaining guide into the hole so that the tubing is kept in place in the cap.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Flush the seal wash system, using fresh seal wash liquid (see Flushing the Seal Wash System).