Items required
Fresh solvent
Observe the following guidelines
The following guidelines refer to conventional use of the Vanquish system as recommended in this manual, such as using fresh high-quality solvents (minimum UHPLC-grade or LC/MS-grade) that are replaced frequently:
Regularly flushing the pump with fresh solvent is usually sufficient.
If you use water, acetonitrile, or methanol for your chromatography, flushing the pump once a week is an appropriate interval.
Mobile phases containing salts, buffering agents and/or other additives are inherently prone to organic contamination and/or biodegradation. When using non-volatile buffers, observe the following:
Mobile phases must be filtered before use.
For continuous trouble-free operation, the system must be flushed with, for example, > 60 % isopropanol or ethanol as a weekly procedure.
Adapt the interval to the solvents in use.
TIP
With persistent contamination, for example, if ghost peaks appear reproducibly in your blank chromatogram without injecting a sample, see Flushing the Pump with Persistent Contamination Only.