Parts and tools required
Seal wash reservoir assembly, including reservoir, reservoir cap, cap plugs, and retaining guide for the seal wash line
Seal wash line (silicone tubing) for connection to the seal wash pump; the line is pre-installed to the seal wash outlet of the metering device head
Seal wash line (silicone tubing) for connection to the seal wash reservoir
TIP
To avoid the formation of air bubbles in liquid lines, always place reservoirs in the solvent rack on top of the system stack.
No. | Description |
---|---|
1 | Seal wash reservoir |
2 | Seal wash lines in the autosampler (thick tubing), with |
2a | Wash line from reservoir to inlet port |
2b | Seal wash inlet port (right tubing connector) |
2c | Wash line from inlet port to metering device head |
2d | Wash line to seal wash pump |
3 | Metering device head |
4 | Seal wash pump (peristaltic pump) |
5 | Seal wash lines in the pump (thin tubing), with: |
5a | Wash lines from seal wash pump to pump head (binary pump, dual pump: to left pump head) |
5b | Isocratic pump, quaternary pump: Wash line from pump head to seal wash detector Binary pump, dual pump: Wash line from left pump head to right pump head |
5c | Binary pump, dual pump: Wash line from right pump head to seal wash detector |
6 | Seal wash detector (drop detector) |
Preparations
Rinse the seal wash reservoir thoroughly. Use a high-purity solvent for rinsing.
Follow these steps
Setting up the seal wash system comprises the following steps:
Engaging the peristaltic tubing
Connecting the seal wash line between autosampler and pump
Routing the seal wash line to the solvent rack
Connecting the seal wash line to the seal wash reservoir
Flushing the seal wash system
Follow the steps in the respective sections.
Engaging the Peristaltic Tubing
In the pump, engage the peristaltic tubing (PharMed™ tubing) in the seal wash pump:
No. | Description |
---|---|
1 | Pump lever |
2 | Pump rotor |
3 | Tubing holder (peristaltic tubing) |
4 | Tubing connector |
5 | Seal wash line from autosampler (silicone tubing) |
6 | Peristaltic tubing |
7 | Seal wash tubing to pump head (silicone tubing) |
Press down the lever on the seal wash pump and hold it in that position with one hand.
With your other hand, insert the tubing between the lever and the rotor and wind it around the rotor.
Release the lever.
Check that the peristaltic tubing is inserted properly in the tubing holders. If it is not, push the tubing into the holders. Make sure that the tubing is not pinched or squeezed in the holders.
Check that the right tubing connector rests on the tubing holder. If it does not, push the tubing connector onto the holder.
Connecting the Seal Wash Line between Autosampler and Pump
In the autosampler, locate the seal wash line (clear silicone tubing) to the pump:
Single split sampler: The seal wash inlet port is located on the top enclosure frame (right tubing connector). The seal wash line to the pump is interconnected to the seal wash inlet port.
Dual split sampler: The seal wash inlet port is located on the right enclosure frame (top tubing connector). The seal wash line to the pump interconnects seal wash inlet and outlet port.
Disconnect the seal wash line from the seal wash inlet port.
Route the seal wash line from the seal wash outlet in the autosampler through the tubing guides to the peristaltic tubing in the pump.
Single split sampler: The seal wash outlet is located on the bottom end of the metering device.
Dual split sampler: The seal wash outlet is located on the right enclosure frame (bottom tubing connector).
In the pump, connect the seal wash line to the tubing connector on the free end of the peristaltic tubing.
No. | Description |
---|---|
A | Single split sampler: Seal wash connections on the top enclosure frame |
B | Dual split sampler: Seal wash connections on the right enclosure frame |
1 | Seal wash inlet port |
1a | Seal wash line to reservoir |
2 | Seal wash outlet |
2a | Seal wash line to pump |
Routing the Seal Wash Line to the Solvent Rack
Locate the seal wash line for connection from the autosampler to the seal wash reservoir.
Connect the seal wash line on the autosampler:
Single split sampler: Connect the line to the seal wash inlet port on the top enclosure frame (right tubing connector).
Dual split sampler: Connect the line to the seal wash inlet port on the right enclosure frame (top tubing connector).
Route the seal wash line from the autosampler to the solvent rack through the tubing guides in the autosampler and all modules above the autosampler in the system stack.
Route the seal wash line through the guide hole in the solvent rack and fix it in the appropriate tubing guide.
No. | Description |
---|---|
A | Guide hole (solvent rack) |
1+2 | Not to be used for seal wash line; reserved for other tubing |
3 | Tubing guide for seal wash line |
4 | Not to be used for seal wash line; reserved for other tubing |
Connecting the Seal Wash Line to the Seal Wash Reservoir
Mind the requirements outlined in Choosing the Seal Wash Liquid.
No. | Description |
---|---|
1 | Wash line |
2 | Retaining guide |
3 | Reservoir cap |
4 | Cap plugs |
Feed the wash line through the retaining guide.
Feed the wash line through an open hole in the cap of the wash reservoir. The retaining guide keeps the tubing in place in the reservoir.
Close any open holes in the reservoir cap with cap plugs.
Fill the wash reservoir with wash liquid.
Tighten the reservoir cap hand-tight. Press the retaining guide into the hole in the reservoir cap so that the tubing is kept in place in the cap.
Place the wash reservoir in the solvent rack.
Position the wash lines straight in the tubing guides.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Flushing the Seal Wash System
To flush the seal wash system, follow the procedure in Flushing the Seal Wash System.