This section provides information on messages with code numbers 5000 through 5999. These messages are specific to the autosampler.
The table lists the most frequently observed messages for the autosampler.
Message and Code | Description and Remedial Action |
---|---|
|
xx = two-digit to four-digit code number When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support. |
| An internal error has occurred. Perform a self-test. |
| Loosen the shipping lock screw above the needle unit. See Unlocking the Needle Unit. Check that the horizontal needle drive can be shifted easily. If obstacles are present in the way of the needle drive, remove the obstacles. |
| Check that the sample loop is installed correctly. See the steps for installation of the sample loop in Sample Loop Maintenance. |
| The autosampler failed the self-test. Check for additional messages that specify the issue. Contact Thermo Fisher Scientific Technical Support for assistance. |
| Rotate the carousel and check that it can be moved easily. If obstacles are present in the way of the carousel, remove the obstacles. |
| Check if a sample rack or well plate is installed in this segment in the carousel. Check that it is placed correctly (see Loading the Carousel) and correct the position, if required. |
| Check if a vial or well plate is present at the specified position. Place a sample container at the specified position (see Loading the Carousel). Check the sample positions defined in the sample sequence. Specify a different position, if necessary. |
| Verify that the needle seat is installed. Install or replace the needle seat and needle unit, if necessary (see Replacing Needle Seat and Needle Unit). |
| Check if the correct sample rack or well plate is configured, and the correct sample position is defined in the sequence. Consider the number of positions of the configured sample rack or well plate as well as the respective well plate height. |
| Verify that the correct sample rack or well plate is configured. Consider the height of the sample rack or well plate. |
|
Bottom sensing is not operational with the current septum. Use supported septum or turn off bottom sensing. For supported vial caps and well plate covers, see Recommendations on Vial Caps and Well Plate Covers. |
| With X.X = the number of minutes With autosamplers that come with an insulation (loop) cover, the cover must be installed to use sample compartment thermostatting. If the cover is not installed properly, thermostatting cannot be enabled or will be turned off automatically to protect the interior components from high humidity and extensive condensation when thermostatting the sample compartment. If the message appears although the cover is mounted, verify that the upper part of the cover is firmly installed (where the magnet is located). You can monitor in the Instrument Audit Trail if the cover is detected properly as you remove and install it. |
| With autosamplers that come with an insulation (loop) cover, the cover must be installed to use sample compartment thermostatting. If the cover is not installed properly, thermostatting cannot be enabled or will be turned off automatically to protect the interior components from high humidity and extensive condensation when thermostatting the sample compartment. If the message appears although the cover is mounted, verify that the upper part of the cover is firmly installed (where the magnet is located). You can monitor in the Instrument Audit Trail if the cover is detected properly as you remove and install it. |
|
With X.X = the number of minutes The insulation cover mounting bracket has been removed while the temperature control was turned on. The insulation cover must be installed to use sample compartment thermostatting. Reinstall or replace the insulation cover mounting bracket as described in Replacing Needle Seat and Needle Unit and install the insulation cover as described in Installing the Insulation Cover. |
|
The insulation cover mounting bracket has been removed while the temperature control was turned on. Reinstall or replace the insulation cover mounting bracket as described in Replacing Needle Seat and Needle Unit and install the insulation cover as described in Installing the Insulation Cover. |
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When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support. |
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When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support. |
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xx = the number of seconds before autosampler is shut down Keep both autosampler doors closed when temperature control is on. |
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Close both autosampler doors and restart temperature control. |
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xx = the affected fan When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support. |
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When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support. |
| Pressure sensor calibration is not possible because system pressure is too low. 1. Visually inspect the system for possible leakage, damaged parts or open connections. 2. Purge the autosampler: Run the script: C04 - Purge Sampler. 3. Check for leakage or air in the system: Run the script: D02 - Test System Tightness (What Should Be Tested = Sampler). 4. Repeat pressure sensor calibration: Run the script: C05 - Adjust System Pressure Sensors. |
| You tried to download the wrong firmware. Select a Vanquish Neo autosampler firmware file for download. |
| Check for additional messages that specify the issue. |
| The autosampler was interrupted during a script, such as A06 - Change Fluidics / Workflow. Repeat the script. |
| With xx = the serial number of the pump Check that all System Interlink cables on the Vanquish system are properly connected. |
| Execute the SelfTest command or turn off the autosampler and turn on again to repeat validation. |
| Run the following script and follow the instructions: D02 - Test System Tightness (What Should Be Tested = Sampler). |
| The autosampler is not ready because, for example, a self-test is running or the autosampler is still busy processing a previous command. Retry when the autosampler is ready. |