This section provides information on messages with code numbers 5000 through 5999. These messages are specific to the autosampler.

The table lists the most frequently observed messages for the autosampler.

Message and Code

Description and Remedial Action

Unexpected module behavior. Code xx

xx = two-digit to four-digit code number

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Code 5002 Command rejected – module is in error state.

An internal error has occurred. Perform a self-test.

Code 5010 Horizontal needle drive blocked. Loosen transport lock completely.

Loosen the shipping lock screw above the needle unit. See Unlocking the Needle Unit. Check that the horizontal needle drive can be shifted easily. If obstacles are present in the way of the needle drive, remove the obstacles.

Code 5011 Vertical needle drive blocked. Check for correct installation of sample loop.

Check that the sample loop is installed correctly. See the steps for installation of the sample loop in Sample Loop Maintenance.

Code 5012 Selftest failed.

The autosampler failed the self-test. Check for additional messages that specify the issue. Contact Thermo Fisher Scientific Technical Support for assistance.

Code 5013 Carousel blocked. Check for obstructions with racks or sample containers.

Rotate the carousel and check that it can be moved easily. If obstacles are present in the way of the carousel, remove the obstacles.

Code 5014 Rack not found. Check for correct rack position in the carousel and sample sequence.

Check if a sample rack or well plate is installed in this segment in the carousel. Check that it is placed correctly (see Loading the Carousel) and correct the position, if required.

Code 5015 Vial not found. Check for correct vial position in the rack and sample sequence.

Check if a vial or well plate is present at the specified position. Place a sample container at the specified position (see Loading the Carousel). Check the sample positions defined in the sample sequence. Specify a different position, if necessary.

Code 5016 Needle seat not found. Install needle seat.

Verify that the needle seat is installed. Install or replace the needle seat and needle unit, if necessary (see Replacing Needle Seat and Needle Unit).

Code 5017 Needle hit an unexpected object due to incorrect sample rack or well plate configuration.

Check if the correct sample rack or well plate is configured, and the correct sample position is defined in the sequence. Consider the number of positions of the configured sample rack or well plate as well as the respective well plate height.

Code 5018 Vial pusher hit an object that is higher than expected. Check the rack and/or well plate configuration.

Verify that the correct sample rack or well plate is configured. Consider the height of the sample rack or well plate.

Code 5020 Septum too strong/tight for bottom detection. Check documentation for supported consumables.

Bottom sensing is not operational with the current septum. Use supported septum or turn off bottom sensing. For supported vial caps and well plate covers, see Recommendations on Vial Caps and Well Plate Covers.

Code 5021 Insulation cover removed while temperature control is on. Replace cover or temperature control will shut down in X.X minutes.

With X.X = the number of minutes

With autosamplers that come with an insulation (loop) cover, the cover must be installed to use sample compartment thermostatting. If the cover is not installed properly, thermostatting cannot be enabled or will be turned off automatically to protect the interior components from high humidity and extensive condensation when thermostatting the sample compartment.

If the message appears although the cover is mounted, verify that the upper part of the cover is firmly installed (where the magnet is located). You can monitor in the Instrument Audit Trail if the cover is detected properly as you remove and install it.

Code 5022 Temperature control shut down due to missing loop cover.

With autosamplers that come with an insulation (loop) cover, the cover must be installed to use sample compartment thermostatting. If the cover is not installed properly, thermostatting cannot be enabled or will be turned off automatically to protect the interior components from high humidity and extensive condensation when thermostatting the sample compartment.

If the message appears although the cover is mounted, verify that the upper part of the cover is firmly installed (where the magnet is located). You can monitor in the Instrument Audit Trail if the cover is detected properly as you remove and install it.

Code 5023 Insulation Loop Cover Bracket removed while temperature control is on. Replace frame or temperature control will shut down in X.X minutes.

With X.X = the number of minutes

The insulation cover mounting bracket has been removed while the temperature control was turned on.

The insulation cover must be installed to use sample compartment thermostatting. Reinstall or replace the insulation cover mounting bracket as described in Replacing Needle Seat and Needle Unit and install the insulation cover as described in Installing the Insulation Cover.

Code 5024 Insulation Loop Cover Bracket missing.

The insulation cover mounting bracket has been removed while the temperature control was turned on.

Reinstall or replace the insulation cover mounting bracket as described in Replacing Needle Seat and Needle Unit and install the insulation cover as described in Installing the Insulation Cover.

Code 5029 Carousel home sensor failure.

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Code 5030 Carousel motor failure or blockade.

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Code 5032 Both doors not closed while temperature control is on. Close both doors or temperature control will shut down in xx minutes.

xx = the number of seconds before autosampler is shut down

Keep both autosampler doors closed when temperature control is on.

Code 5033 Temperature control shut down due to open door.

Close both autosampler doors and restart temperature control.

Code 5035 Temperature control shut down due to xx fan failure.

xx = the affected fan

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Code 5037 Backup memory error. Module not operational.

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Code 5039 Calibration process refused, pressure below 100 bar.

Pressure sensor calibration is not possible because system pressure is too low.

1. Visually inspect the system for possible leakage, damaged parts or open connections.

2. Purge the autosampler: Run the script: C04 - Purge Sampler.

3. Check for leakage or air in the system: Run the script: D02 - Test System Tightness (What Should Be Tested = Sampler).

4. Repeat pressure sensor calibration: Run the script: C05 - Adjust System Pressure Sensors.

Code 5040 Model – Firmware Mismatch! This firmware only supports VN-A10 models! Download appropriate firmware!

You tried to download the wrong firmware. Select a Vanquish Neo autosampler firmware file for download.

Code 5041 Abort error occurred. Command sequence aborted.

Check for additional messages that specify the issue.

Code 5042 Vanquish Neo sampler detected unknown inject valve bypass position. Set correct position and run self-test command.

The autosampler was interrupted during a script, such as A06 - Change Fluidics / Workflow. Repeat the script.

Code 5043 Vanquish Neo Pump not found on system interlink. Cannot receive pressure from pump SN xx.

With xx = the serial number of the pump

Check that all System Interlink cables on the Vanquish system are properly connected.

Code 5044 Validation of vial bottom detection failed.

Execute the SelfTest command or turn off the autosampler and turn on again to repeat validation.

Code 5045 Could not pressurize while delivering solvent. Check for air or leakages in system.

Run the following script and follow the instructions: D02 - Test System Tightness (What Should Be Tested = Sampler).

Code 5099 The device is busy.

The autosampler is not ready because, for example, a self-test is running or the autosampler is still busy processing a previous command. Retry when the autosampler is ready.