This section provides information on messages with code numbers 4000 through 4999. These messages are specific to the pump.

Additional Source Identification

To narrow down the source of a problem, check the message in the chromatography data system for additional information:

Default Name in Data System

Description

PumpModule.Pump

The message relates to the entire pump module, including flowmeter (with system pressure sensor).

PumpModule.Pump
Left Block (WP)

The message relates to the following:

  • Left pump block (A)

  • Components in the left pump block, for example, the pump head

  • Components on the left side of the left pump head, for example, the left pressure sensor in the pump head or the working piston, delivering %A.

PumpModule.Pump
Left Block (EP)

The message relates to the following:

  • Left pump block (A)

  • Components in the left pump block, for example, the pump head

  • Components on the right side of the left pump head, for example, the right pressure sensor in the pump head or the equilibration piston, delivering %A

PumpModule.Pump
Right Block (WP)

The message relates to the following:

  • Right pump block (B)

  • Components in the right pump block, for example, the pump head

  • Components on the left side of the right pump head, for example, the left pressure sensor in the pump head or the working piston, delivering %B

PumpModule.Pump
Right Block (EP)

The message relates to the following:

  • Right pump block (B)

  • Components in the right pump block, for example, the pump head

  • Components on the right side of the right pump head, for example, the right pressure sensor in the pump head or the equilibration piston, delivering %B

Pump Messages

The table lists the most frequently observed messages for the pump.

Message and Code

Description and Remedial Action

Unexpected module behavior. Code xx

xx = two-digit to four-digit code number

When the message appears, write down the message code and turn off the module. Wait for 5 seconds and turn on the module again. If the message appears again, contact Technical Support.

Module malfunction detected. Code xx

xx = two-digit to four-digit code number

When the message appears, write down the message code. Turn off the module and contact Technical Support.

Code 4098 Upper pressure limit exceeded

Check the flow path for blockage. Run the script: D01 - Test System Back Pressure.

Code 4099 Pressure fallen below lower limit.

Check the following causes:

  • One or more solvent reservoirs are empty. Run the script: B01 - Change Liquids / Solvents (Refresh Only = On).

  • Air bubbles may be trapped in the solvent lines. Check the solvent line filters. Purge the pump.

  • With the applied flow, the pump cannot build up enough pressure to reach the lower pressure limit. Adapt the lower pressure limit or increase the flow.

  • Leakage may be present in the system. Run the script: E04 - Detailed Leak test (Pump).

  • A check valve may be contaminated or defective. Clean or replace the check valves. See Maintaining the Pump Head Check Valves.

Code 4100 Purge pressure limit exceeded

A pressure of more than 5 MPa (50 bar) built up after a purge cycle has started.

  • Run the script: D01 - Test System Back Pressure. Take the remedial actions suggested by the script.

  • If the message appears together with a message regarding the flowmeter filter backpressure (code 4533), replace the filter (see Replacing the Flowmeter Filter).

  • A purge valve may be defective. Contact Technical Support.

Code 4101 Not ready. Please run self-test.

The pump is not ready because the self-test was not successful. Repeat the self-test. Run the script: B04 - System Self-Test.

Code 4112 Self-test failed because of fluctuating pressure.

Changes in pressure have occurred during the self-test. Wait until the system pressure is down or depressurize the system and repeat the self-test. Run the script: B04 - System Self-Test.

Code 4113 Self-test failed because pressure is out of range.

The pressure has not been down to zero during the self-test. Verify that the pressure sensor cables are properly connected. Wait until the system pressure is down or depressurize the system and repeat the self-test. Run the script: B04 - System Self-Test.

If the message appears again, the system pressure sensor may be defective. Contact Technical Support.

Code 4114 The device is busy. Please retry after 1 minute.

The pump is not ready because, for example, a self-test or recalibration procedure is running. Retry after 1 minute. If the message appears again, wait until the system pressure is down or depressurize the system and turn the pump off and on again.

Code 4119 Can’t start pump while alarm is on.

An alarm is present, for example, because leakage has been detected. You can restart the pump flow only when the alarm is no longer present. Mute the alarm by pressing the MUTE ALARM button on the pump keypad. Find and eliminate the source for the alarm as suggested for the accompanying message.

Code 4120 The rear seal wash system has run out of wash solution.

This message alerts you only when the drop counter functionality of the seal wash detector is enabled (standard setting).

  • The seal wash reservoir may be empty. Provide new seal wash liquid and run the script: B01 - Change Liquids / Solvents (Rear Seal Wash Liquid, Refresh Only = On). Observe the recommendations for the seal wash liquid in Filling the Seal Wash Reservoir.

  • Inspect the seal wash lines across the entire flow path for indications of blockage or leakage. All lines must be properly connected and routed. Replace the seal wash lines as necessary. See Seal Wash System Maintenance.

  • Check whether liquid escapes from the connections of the seal wash system or under the pump heads. See Testing the Seal Wash System for Leakage.

  • Inspect the peristaltic tubing for indications of blockage or damage. Replace the tubing as necessary. See Replacing the Peristaltic Tubing in the Seal Wash Pump.

  • Verify that the peristaltic tubing is properly inserted in the seal wash pump and that the lever of the pump is not blocked.

  • Verify that the seal wash tubing is connected to the correct port on the seal wash detector (port labelled in; see picture in Testing the Seal Wash System for Leakage).

  • Inspect the electrodes of the seal wash detector for contamination or damage. Replace the seal wash detector as necessary. See Replacing the Seal Wash Detector.

Code 4121 Piston seal leakage has exceeded the recommended limit.

This message alerts you only when the drop counter functionality of the seal wash detector is enabled (standard setting).

Test the pump for leakage. Run the script: E04 - Detailed Leak test (Pump). When the piston seals are leaky, replace the seals. See Replacing the Piston Seal.

Code 4122 The rear seal leak sensor detects drops constantly.

This message alerts you only when the drop counter functionality of the seal wash detector is enabled (standard setting).

  • Verify that the liquid flows off properly into the drain system, through the drain port at the bottom right of the leak tray.

  • Inspect the bottom side of the seal wash detector: Remove the seal wash detector assembly from the pump and disconnect the detector from the funnel. Dry the bottom side with a cloth or tissue. Inspect the detector electrodes. If they are clean and undamaged, reassemble the detector and the funnel and reinstall the assembly in the pump. See Replacing the Seal Wash Detector.

  • If the message appears again, the seal wash detector may be defective. Replace the seal wash detector. See Replacing the Seal Wash Detector.

Code 4125 Degasser malfunction

The vacuum level monitoring function detected insufficient degasser vacuum.

Update the firmware to version 2.41 or later.

Code 4127 The pump drive is still in undock position. Please execute a dock command.

This message alerts you during pump maintenance (for example, when replacing the piston seals or pistons) when you try to start the pump while the pump head is not yet in the position for operation.

Return the pump head into the position for operation: On the keypad, the LEDs next to the FLOW button indicate which pump head is in the position for maintenance (LED is flashing green). Verify that this pump head is selected (LED next to the SELECT button is green) and press the DOCK button. When the pump is in the position for operation, the LED next to the FLOW button stops flashing.

Code 4148 Can’t perform this command while the flow is on.

You tried to run a self-test while the flow rate was not yet down to zero. Turn off the flow and repeat the command.

Code 4152 Pressure sensor malfunction. Check cable connection and retry.

Verify that the cable of the pressure sensor for which the message appears is properly plugged into the connection port. Restart the flow.

If the message appears again, the pressure sensor may be defective:

  • If the message appears for the pressure sensor of a pump head, replace the pump head.

  • If the message appears for the system pressure sensor, contact Technical Support.

Code 4158 Out of eluent X.

This message alerts you only if you are monitoring the solvent consumption. The message indicates for which solvent the reservoir is empty. Provide new solvent. Run the script: B01 - Change Liquids / Solvents.

Code 4161 Pressure recalibration deviates by xx bar.

The pump pressure was not down to zero during the self-test. Wait until the pressure is down and repeat the test. Run the script: B04 - System Self-Test.

Code 4208 System pressure too high. Please relieve pressure and retry.

Follow these steps:

1. Verify that the pressure sensor cables are properly connected. Reconnect the cables if necessary.

2. Check the flow path for blockage. Run the script: D01 - Test System Back Pressure.

3. Run a self-test. Run the script: B04 - System Self-Test.

If the message persists, the system pressure sensor may be defective. Contact Technical Support.

Code 4209 Could not build up enough pressure. Please check the fitting plug.

The pump could not build up enough pressure during pressure sensor calibration. This may be caused by the following:

  • Air may be trapped in the system. Purge the pump. Run the script: C02 - Purge Pump.

  • Leakage may be present. Run the script: E04 - Detailed Leak test (Pump).

Code 4213 Head pressure does not match system pressure. Execute the self-test or perform pressure recalibration.

1. Inspect the flow path from the pump head to the flowmeter inlet for signs of blockage, especially the Viper ends. If you suspect blockage, replace the capillary.

2. Run a self-test. Run the script: B04 - System Self-Test.

3. If the message persists, perform pressure recalibration. Run the script: C05 - Adjust System Pressure Sensors.

4. If the message appears again, contact Technical Support.

Code 4220 Pump head not recognized. Please plug in the pressure sensor cable.

1. Connect the pressure sensor cable from the pump head to the Pressure connector. If the cable is connected, verify that it is properly seated.

2. If the problem persists, the pump head may be defective. Contact Technical Support or replace the pump head (see Replacing the Pump Head).

Code 4231 Pressure sensor not recognized. Please plug in the sensor cable.

Verify that the cable of the pressure sensor for which the message appears is properly plugged into the connection port. Restart the flow.

If the message appears again, the pressure sensor may be defective:

  • If the message appears for the pressure sensor of a pump head, replace the pump head (see Replacing the Pump Head).

  • If the message appears for the system pressure sensor, the system pressure sensor may be defective. Contact Technical Support.

Code 4232 The identification number of the XX has changed.

XX is the left pump head or right pump head

The message appears when a pump head has been replaced or when the left and right pump heads have been interchanged, for example, accidentally during maintenance/service. The message is for informational purposes only. No action is required.

Code 4234 The flow was stopped due to a request from another module.

The pump flow was stopped because of a problem with another module. Check the messages from other modules.

Code 4238 Mainboard initialization failed.

Main board initialization has not been successful.

1. This may be a temporary error. Turn off the module. Wait for 5 seconds and turn on the module again.

2. If the message appears again, update the firmware.

3. If the message persists, the main board may be defective. Contact Technical Support.

Code 4503 The pressure exceeded the absolute limit.

Test the flow path for blockage. Run the script: D01 - Test System Back Pressure.

Code 4504 NC drive initialization error.

Repeat the self-test. Run the script: B04 - System Self-Test.

Code 4508 Piston drive home sensor remains dark.

Repeat the self-test. Run the script: B04 - System Self-Test.

Code 4512 Cannot pressurize due to inlet valve failure or air in system.

A compression phase had to be repeated as the expected pressure was not reached. This can be due to air in the system or because the inlet check valve needs cleaning. However, the pump delivers the correct flow as the following compression phase was successful.

If the message is rare and not accompanied by other messages, for example code 4532 or code 4538, you can tolerate the message. Otherwise:

  • Check the liquid level in the solvent reservoir.

  • Purge the pump. Run the script: C02 - Purge Pump.

  • If the message appears again after purging, clean the inlet check valve (see Cleaning the Check Valves (Pump Head)).

  • Only use the genuine solvent lines authorized by Thermo Fisher Scientific for this pump.

Code 4513 Home position deviation XX. Please execute self-test.

XX is the value for the deviation.

Make sure that you use the recommended seal wash liquid (see Filling the Seal Wash Reservoir) and run a self-test. Run the script: B04 - System Self-Test.

Code 4514 The user defined calibration data of the flow sensor is invalid.

The calibration data for the selected solvent is incomplete. For example, because the calibration procedure has been interrupted unexpectedly.

Repeat the calibration for the selected solvent (see Adding Custom Solvents to the Calibration Table).

Code 4517 Residual pressure too high for flow autozero.

Stop the flow and wait until the pressure has dropped.

Code 4519 Calibration table full.

Delete a solvent from the solvent calibration table to free up space. On the script panel, go to Solvent Calibration. Run the script: M34 - Delete Solvent Calibration Data.

Code 4520 Flowmeter memory unavailable.

Check that the flowmeter is correctly inserted.

Code 4522 Unknown solvent.

The solvent you selected is not available in the solvent calibration table. Possible reasons are: The solvent was removed from the calibration table. The flowmeter was replaced, and a solvent (with the same name) has not been calibrated and added to the configuration of the new flowmeter.

Select a solvent from the solvent calibration table or add the solvent to calibration table (see Adding Custom Solvents to the Calibration Table).

Code 4523 Drift rate too high for flow autozero.

The flow is not stable.

1. Wait until the system pressure is down to zero.

2. Purge the pump to remove residual air.

3. When the pump has been powered on for at least 6 hours, repeat autozero.

Code 4526 Command sequence error.

The commands were executed in a wrong or unexpected order. Check the command script in your method for errors.

Code 4529 The pump capillary connections are setup for solvent calibration. Normal operation is not possible.

A calibration attempt has been interrupted. Use the solvent calibration scripts to either complete the calibration or return the pump to the original capillary configuration as required by the application.

Code 4531 Cannot deliver flow at this pressure.

The pump cannot deliver the requested flow rate at the resulting pressure. Adapt the application setup. For example, reduce the flow rate. See Dependency of Available Pump Pressure and Flow Rate.

Code 4532 Cannot regulate flow due to air in system or inlet valve failure.

One or more compression phases were unsuccessful. The pump could not deliver the correct flow. This can be due to air in the system or because the inlet check valve needs cleaning. The message appears together with message code 4512.

  • Check the liquid level in the solvent reservoir.

  • Purge the pump. Run the script: C02 - Purge Pump.

  • If the message appears again after purging, clean the inlet check valve (see Cleaning the Check Valves (Pump Head)).

  • Only use the genuine solvent lines authorized by Thermo Fisher Scientific for this pump.

Code 4533 Elevated filter backpressure. Consider replacing the flowmeter filter soon.

The message appears to inform you before an actual problem occurs. The message indicates that there may be a problem with the solvents, for example, algae growth.

Consider replacing the flowmeter filter (see Replacing the Flowmeter Filter). Observe the recommendations in Use of Solvents and Additives.

Code 4534 System pressure exceeded maximum pressure during purge.

1. On the flowmeter outlet, remove the capillary to the autosampler and retry.

2. Depends as follows:

  • The error is no longer present: The autosampler fluidics or the capillary to the autosampler may be clogged. As applicable, eliminate the source for the clogging and/or replace the capillary from the flowmeter to the autosampler.

  • The error persists: In the flowmeter, the Y-piece or the restrictor capillaries may be clogged. Contact Technical Support.

Code 4536 Solvent calibration failed.

Capillary connections or plugs on the pump heads or flowmeter may not be connected properly or are not tight.

1. Reconnect or retighten as necessary.

2. Repeat the calibration.

Code 4538 Flow too low – check for leaks.

The pump cannot deliver the selected flow rate. The message may appear alone or together with message code 4512.

  • Message is accompanied by message code 4512: Follow the remedial actions suggested for the accompanying message. If this does not remedy the situation, there may be leakage in the system. Find and eliminate the source for the leakage (see Testing for Liquid Leaks).

  • Message appears alone: There may be leakage in the system. Find and eliminate the source for the leakage (see Testing for Liquid Leaks).

Code 4539 Flow too high.

The pump cannot reduce the flow rate to the nominal flow.

  • Message appears when the set flow rate is close to zero (for example, when turning off the pump flow): Adjust the pressure and flow sensors. Run the scripts: C05 - Adjust System Pressure Sensors and C01 - Adjust Pump Flow Sensor Offsets.

  • Message appears in other situations: Contact Technical Support.