The pump can only deliver accurate flow rates if the solvent that you plan to use has been calibrated. Calibrated solvents can be selected in the script A01 - Set Pump Solvent Types or in the solvent calibration table. The most common solvent types and mixtures were calibrated at the factory:

  • Water, 100%

  • Acetonitrile, 100%

  • Mixture of acetonitrile and water, 80:20 (v:v)

  • Mixture of methanol and water, 90:10 (v:v)

If you want to use other solvents, calibrate them once to add them to the configuration. Calibration is recommended for a solvent type differing by > 5% (volume-by-volume) from one of the above.

Examples

Example 1

Solvent

Action

A

98:2 (v:v), water:acetonitrile plus 0.1% formic acid

Use the calibration data for 100% water.

B

80:20 (v:v), acetonitrile:water plus 0.1% formic acid

Use the calibration data for 80:20% acetonitrile.

Example 2

Solvent

Action

A

95:5 (v:v), water:dimethyl sulfoxide plus 0.1% formic acid

Use the calibration data for 100% water.

B

75:20:5 (v:v:v), acetonitrile:water:dimethyl sulfoxide plus 0.1% formic acid

Use the calibration data for 80:20% acetonitrile.

Example 3

Solvent

Action

A

100% water plus 0.1% formic acid

Use the calibration data for 100% water.

B

90:10 (v:v), acetonitrile:water plus 0.1% formic acid

Calibration is required. See further down.

General Notes

  • Solvent calibration is per channel.

  • If you want to use a solvent in both channels, you must perform the calibration twice to calibrate the solvent for each channel separately.

  • The actual calibration is automated and runs unattended. Prior to starting the script for the actual calibration, you must setup and configure the pump for calibration and provide user input. After completion, you must return the pump to the original capillary configuration and reconnect all capillaries and the system as required by the application.

  • Calibration scripts guide you through all steps of the process.

  • The time required for the actual calibration to complete is approximately 100 minutes. In addition, allow for 30 minutes beforehand to prepare the calibration setup and 30 minutes afterward to restore the operational configuration.

Parts and Items required

  • Solvent suitable for purging to flush out harmful substances

  • Solvent for which you want to perform the calibration

  • Viper fitting plug (3 plugs)

Follow these steps

  1. On the script panel, set the filter to Solvent Calibration.

  2. Run and follow the solvent calibration scripts one after the other.

  3. After the operational configuration has been restored, test the pump for leakage. Run the script: E04 - Detailed Leak test (Pump).

Calibration Results

  • Calibration successful: The solvent is added to the solvent calibration table and is available for selection.

  • Error occurred during calibration: From the calibration scripts, either complete the calibration or restore the operational configuration.