The flow sensor offset adjustment script measures the sensor signal at zero flow and saves the result so that the flow signal can be corrected accordingly. This will remove the offset shift that has occurred since the calibration in the factory.
Adjustment not required
Adjusting the flow sensor offsets is not required when the retention times are stable or in the expected range. Do not adjust the flow sensor offsets in these situations:
While a scientific study or measurement series is running.
During a series of measurements that are expected to give comparable results.
Very frequently, for example, every day.
Adjustment may be required
For accurate and comparable measurement results adjusting the flow sensor offsets may be required in these situations:
When retention times are noticeably different from the expected range (shifted). This may lead to missing peaks.
When retention times vary considerably compared to previous measurements.
Estimated, adjusting the flow sensor offsets will be required once every 1 to 3 months, specifically when running < 2 µL/min analysis.
Follow these steps
Observe the following before you begin:
The system must have been on for a minimum of 5 hours. Only then, the adjustment will be possible.
Recommendation: Perform the adjustment using the solvents of your application.
Run this adjustment script: C01 - Adjust Pump Flow Sensor Offsets.
When the adjustment is complete, allow some equilibration time for optimum retention time stability, for example, one blank run.