Follow the steps below to replace the two wash lines between the needle wash reservoirs and the needle wash pump inlet ports of the autosampler.

When

Wash line is clogged or damaged

Parts and additional items required

  • Fresh needle wash liquid

  • Replacement wash lines labeled with SWP (red) and WWP (green)

Tools required

Tubing cutter (optional)

TIP

If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.

Preparations

  1. Prepare fresh needle wash liquid.

  1. Empty the washing system for the outside of the needle:

  1. Unscrew the caps of the needle wash reservoirs.
  2. Together with the cap, remove the wash lines (labeled WWP and SWP) from the reservoir.
  3. When removing the lines from the tubing guides, be careful not to pull on other tubing in the guides.
  4. Place the wash lines in a waste reservoir to empty them.

Follow these steps

  1. Remove the retaining guide from the wash lines.

  2. Pull the lines out of the reservoir caps.

  3. Disconnect the wash lines from the needle wash inlets ports on the needle wash pump.

  4. Figure: Disconnecting the wash lines from the needle wash pump inlet
  5. Connect the replacement wash lines to the needle wash pump inlet ports:

  1. Push the rigid tube into the connector.
  2. Push the silicon tube over the connector.
  1. Route the wash lines from the autosampler to the solvent rack, through the tubing guides in the autosampler and all modules above the autosampler in the system stack.

  2. Route the wash lines through the guide hole in the solvent rack and push them into one or more of the tubing guides.

Guide hole and tubing guides in the solvent rack
Figure: Guide hole and tubing guides in the solvent rack

No.

Description

A

Guide hole (solvent rack)

1-4

Tubing guides

  1. Feed each wash line through a retaining guide.

  2. Feed each wash line through an open hole in a cap for a reservoir. The retaining guide keeps the solvent line in place in the reservoir.

  3. Close any open holes in the reservoir caps with the cap plugs.

  4. Slide a filter holder with adapter onto each wash line.

  1. Fill the needle wash reservoirs with fresh needle wash liquid and restart the needle wash system. For details, see Filling the Needle Wash Reservoirs.