NOTICE

Particles and air bubbles can impair the system functionality

Some components of the system are filled with isopropanol when they were stored or when they are shipped from the manufacturing site.

  • To flush out air bubbles (and isopropanol) before operating the system for the first time, run the script: A02 - Auto Start Up.
  • To flush out isopropanol, use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent.

When

Before using the system for the first time

Solvent and seal wash liquid volumes required

Needed liquid

Consumption during script execution: A02 - Auto Start Up

Consumption during analysis

Liquid requirements

Solvent

Max. 10 mL per solvent reservoir

Depends on the flow rate

If operating the system or a component for the first time: Use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent.

In all other cases, see Solvent and Additive Information.

Seal wash liquid

Max. 10 mL

Approx. 250 mL per 14 days

See Filling the Seal Wash Reservoir.

Needle wash liquid volumes required

Consumption during the script: A02 - Auto Start Up

Consumption during analysis

Liquid requirements

Max. 10 mL

Consumption per needle wash reservoir and per injection1:

See Filling the Needle Wash Reservoirs.

SWP

500 µL

WWP

400 µL

W

250 µL

1: The values apply to: 25‑µL sample loop, direct injection (loop in bypass) set in chromatography data system, default wash settings of the chromatography data system.

Note: The consumption depends on the sample loop size, column size, wash time, workflow, and wash speed. If using other settings, the values can be significantly higher.

Follow these steps

  1. Check the liquid level of all reservoirs, especially that of the needle wash reservoirs. Make sure that the amounts are sufficient for the following:

    • Script A02 - Auto Start Up (see the table above)

    • First analysis (see the table above and add the amounts depending on the flow rate of your application).

  2. Close the doors of all modules in the Vanquish Neo system, if not already done.

  3. To set the solvent A/B, run the script: A01 - Set Pump Solvent Types.

  4. Run the script with or without diagnostics (leak test): A02 - Auto Start Up.

    The script flushes the system flow path up to the column inlet capillary, tests the system backpressure, and, optionally, performs a leak test.

  5. To set a separation column type, run the script: A03 - Set Separation Column Type.

  6. To set the separation column properties, run the script: A04 - Set Separation Column Specifications.

  7. To condition the column, run the script: B06 – Condition Column(s).