When

  • After powering up the system and after preparing the system for initial operation

  • After a short-term shutdown

  • After changing the flow rate

  • After changing the temperature setpoints

Follow these steps

System equilibration should include the following:

  1. Warming up (or cooling down) all temperature-controlled devices for a certain time:

Equilibration steps

Duration

Warming up (or cooling down) all temperature-controlled devices in the system to the starting temperature. Temperature-controlled devices can be, for example:

  • Pump flowmeter

  • Sample compartment thermostatting in the autosampler

  • Column compartment and column

5 hours depending on the temperature differences needed for your application

  1. During warming up (or cooling down), do the following depending on the situation:

    • If you use the previously installed column: Equilibrate the column by running the script: B02 – Clean and Equilibrate Column(s).

    • If you installed a new, previously unused, column: To condition new columns, run the script: B06 – Condition Column(s).

  2. Monitor the pump pressure drift and check that the drift is within reasonable limits for the application for 5-24 hours depending on the retention time accuracy needed for your application.