Product component

What you should do...

System/all modules

  • Flow connections:

    Inspect flow connections for signs of:

    • Blockage: Run the script and follow the instructions: D01 - Test System Back Pressure.

    • Salt deposits

    • Air bubbles

      Sonicate the solvents and purge the pump and the autosampler if necessary.

  • Solvents:

    If you use one of the following solvents, proceed as follows:

    • Pure water or an aqueous buffer: To replace the solvent reservoir, run the script: B01 - Change Liquids / Solvents.

    • Buffers or salt solutions: Flush the system thoroughly after use with an appropriate solvent that does not contain buffers or salts. See Purging (Overview).

    • Corrosive solvents or methanol with <10% water: Flush the system with water or other suitable non-corrosive solvents. See Purging (Overview).

Wash systems

  • Check the liquid level.

  • Check if particles, dust, or algae are present in the wash reservoirs.

    If particles, dust, or algae are present, replace the wash reservoirs with clean ones. Run the script: B01 - Change Liquids / Solvents (Refresh Only = On). Ensure that the wash reservoirs are free of detergents or residual chemicals. We recommend rinsing the wash reservoirs with a small quantity of the planned wash liquid.

Autosampler

  • Inspect vials and well plates in the autosampler for cracks or defects.

  • Clean up spills inside the autosampler if necessary.