The autosampler performance is specified as follows:
Type | Specification | |
---|---|---|
Injection method | Split-loop, direct injection or trap-and-elute | |
Number of injection units | 1 | |
Pressure range | 5 - 151 MPa (50 - 1517 bar, 700 - 22002 psi) | |
Injection volume range | 25 µL sample loop (standard) | 0.01 µL - 25 µL, settable in steps of min 0.01 µL |
100 µL sample loop (extended) | 0.01 µL - 100 μL, settable in steps of min 0.01 µL High-volume trapping by multiple draw cycles and trap cycles: up to 500 μL | |
Injection volume accuracy | Typically, ± 0.5% with an injection volume of 5 µL and water | |
Injection volume precision | 0.05 – 0.20 µL | <5.0% |
0.20 – 0.50 µL | <1.5% | |
0.50 – 2.00 µL | <0.5% | |
Above 2 µL | <0.25% | |
Low sample loss features | 2.5 µL out of 3 µL with adequate vials (see Recommendations on Vial Caps and Well Plate Covers) | |
Linearity | r > 0.9999 (caffeine in water) | |
Needle wash | Dip rinse and continuous rinse of external needle surface with 2 independent wash liquids. Rinse of sample loop and internal needle surface with strong wash liquid from the wash port (SWP). Afterward, rinse of sample loop and internal needle surface with weak wash liquid from the metering device (W). | |
Number of needle wash liquids | 2 | |
Dwell volume | With sample loop volume of 25 µL: | |
In inject position | 55 µL | |
In bypass position | 51 nL | |
Carry over | <0.002% with caffeine | |
Barcode reader | Yes | |
Automation Features Barcode Reading | Barcode reading:
| |
Sample compartment thermostatting: Temperature range | 4 - 40 °C, cooling ≥ 23 K below ambient at < 80% relative humidity | |
Temperature accuracy | -2 °C and +4 °C | |
Temperature stability | ± 1 °C | |
Sample capacity | 4 segments useable for sample racks or well plates with SBS footprint format; plus support of vial holders with a capacity of (number of vials x O.D.): 12 x 22.5 mm | |
Vial capacity |
| |
Well plate capacity | 96- and 384-position well plates with SBS footprint | |
Minimum sample required | 2 µL at 1 µL injection volume | |
USB Communication | 1 USB port (USB 2.0, "B" type connector) 1 USB hub with 3 ports (USB 2.0, "A" type connectors) | |
I/O Interface | 2 digital I/O ports (mini DIN), each providing one digital input, and one relay output | |
System Interlink | 2 system interlink ports (RJ45-8 connectors) | |
Materials in the analytical flow path | Titanium, ceramics, PEEK, MP35N, DLC, sapphire, fluoropolymers Note: For information about the seal wash liquid to be used, see Filling the Seal Wash Reservoir. For information about the chemical resistance of materials, refer to the technical literature. | |
Materials in the needle wash flow path | PP (polypropylene), PE, FFPM (perfluoro-elastomer), FFKM, PEEK, PA (polyamide), PK (polyketone), TPE (thermoplastic elastomer) | |
Materials in the seal wash flow path | Titanium, ceramics, PEEK, UHMW PE, PP, PTFE, silicone |