The autosampler performance is specified as follows:

Type

Specification

Injection method

Split-loop, direct injection or trap-and-elute

Number of injection units

1

Pressure range

5 - 151 MPa (50 - 1517 bar, 700 - 22002 psi)

Injection volume range

25 µL sample loop (standard)

0.01 µL - 25 µL, settable in steps of min 0.01 µL

100 µL sample loop (extended)

0.01 µL - 100 μL, settable in steps of min 0.01 µL

High-volume trapping by multiple draw cycles and trap cycles: up to 500 μL

Injection volume accuracy

Typically, ± 0.5% with an injection volume of 5 µL and water

Injection volume precision

0.05 – 0.20 µL

<5.0%

0.20 – 0.50 µL

<1.5%

0.50 – 2.00 µL

<0.5%

Above 2 µL

<0.25%

Low sample loss features

2.5 µL out of 3 µL with adequate vials (see Recommendations on Vial Caps and Well Plate Covers)

Linearity

r > 0.9999 (caffeine in water)

Needle wash

Dip rinse and continuous rinse of external needle surface with 2 independent wash liquids.

Rinse of sample loop and internal needle surface with strong wash liquid from the wash port (SWP). Afterward, rinse of sample loop and internal needle surface with weak wash liquid from the metering device (W).

Number of needle wash liquids

2

Dwell volume
(contribution of the autosampler to the system gradient delay volume)

With sample loop volume of 25 µL:

In inject position

55 µL

In bypass position

51 nL

Carry over

<0.002% with caffeine

Barcode reader

Yes

Automation Features Barcode Reading

Barcode reading:

  • Empty segment detection

  • Rack/well plate verification

  • Inventory management

Sample compartment thermostatting: Temperature range

4 - 40 °C, cooling ≥ 23 K below ambient at < 80% relative humidity

Temperature accuracy

-2 °C and +4 °C

Temperature stability

± 1 °C

Sample capacity

4 segments useable for sample racks or well plates with SBS footprint format; plus support of vial holders with a capacity of (number of vials x O.D.): 12 x 22.5 mm

Vial capacity

  • 54 x 12 mm O.D. vials

  • 96 x 6 mm O.D. vials

  • 96 x 7 mm O.D. vials

  • 96 x 8 mm O.D. vials

  • 16 x 15 mm O.D. vials

  • 9 x 22.5 mm O.D. vials

Well plate capacity

96- and 384-position well plates with SBS footprint

Minimum sample required

2 µL at 1 µL injection volume

USB Communication

1 USB port (USB 2.0, "B" type connector)

1 USB hub with 3 ports (USB 2.0, "A" type connectors)

I/O Interface

2 digital I/O ports (mini DIN), each providing one digital input, and one relay output

System Interlink

2 system interlink ports (RJ45-8 connectors)

Materials in the analytical flow path

Titanium, ceramics, PEEK, MP35N, DLC, sapphire, fluoropolymers

Note: For information about the seal wash liquid to be used, see Filling the Seal Wash Reservoir.

For information about the chemical resistance of materials, refer to the technical literature.

Materials in the needle wash flow path

PP (polypropylene), PE, FFPM (perfluoro-elastomer), FFKM, PEEK, PA (polyamide), PK (polyketone), TPE (thermoplastic elastomer)

Materials in the seal wash flow path

Titanium, ceramics, PEEK, UHMW PE, PP, PTFE, silicone