Follow the steps below to replace the wash line between seal wash reservoir and the seal wash inlet port on the autosampler.
When
Seal wash reservoir line is clogged or damaged
Parts required
Silicone tubing and connectors
Tools required
Tubing cutter
Preparations
Empty the seal wash system:
- Unscrew the cap of the seal wash reservoir.
- Together with the cap, remove the seal wash line from the seal wash reservoir.
- To empty the seal wash line, lift up the lever of the seal wash pump. Release the lever when the line is empty.
Removing the Seal Wash Reservoir Line
Pull the seal wash reservoir line with the tubing connector out of the seal wash inlet port.
Disconnect the seal wash reservoir line, the tubing connector, and the seal wash inlet line. The seal wash line on the metering device head can remain connected.
Disconnect the line from the seal wash reservoir:
- Remove the retaining guide from the wash line.
- Pull the wash line out of the reservoir cap.
No. | Description |
|---|---|
1 | Wash line |
2 | Retaining guide |
3 | Reservoir cap |
4 | Cap plugs |
Connecting the New Seal Wash Reservoir Line
Cut tubing to required length, if applicable.
Connect the seal wash line with a new tubing connector to the seal wash inlet line.
Push the tubing connector with the lines into the seal wash inlet port.
Route the seal wash line from the autosampler to the solvent rack through the tubing guides in the autosampler and all modules above the autosampler in the system stack.
Route the seal wash line through the guide hole in the solvent rack and fix it in one of the tubing guides.
No. | Description |
|---|---|
A | Guide hole (solvent rack) |
1-4 | Tubing guides |
Connect the seal wash reservoir to the line:
- Rinse the seal wash reservoir thoroughly with a high-purity solvent.
- Fill the seal wash reservoir with fresh seal wash liquid.
- Feed the seal wash line through the retaining guide and through an open hole in the cap of the seal wash reservoir. The retaining guide keeps the tubing in place in the reservoir. Close any open holes in the reservoir cap with cap plugs.
Restarting the Seal Wash System
Retighten the reservoir cap hand-tight. The retaining guide must remain in the hole in the reservoir cap. If it does not, press the retaining guide into the hole so that the tubing is kept in place in the cap.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Flush the seal wash system. Run the script: B01 - Change Liquids / Solvents (with: Rear Seal Wash Liquid and Refresh Only = Off.)