Follow the steps below to replace the wash line between seal wash inlet port on the autosampler and the seal wash inlet of the metering device head.

Figure: Position of seal wash inlet line

When

Seal wash inlet line is clogged or damaged

Parts required

Silicone tubing and connectors

Tools required

Tubing cutter

Preparations

  1. Empty the seal wash system:

  1. Unscrew the cap of the seal wash reservoir.
  2. Together with the cap, remove the seal wash line from the seal wash reservoir.
  3. To empty the seal wash line, lift up the lever of the seal wash pump. Release the lever when the line is empty.

Follow these steps

  1. Pull the seal wash inlet line with the tubing connector out of the seal wash inlet on top of the metering device head.

  2. Figure: Disconnecting the seal wash inlet line from the metering device head
  3. Pull the seal wash line with the tubing connector out of the seal wash inlet port.

  4. Figure: Pulling the tubing connector out of the seal wash inlet port
  5. Disconnect the seal wash inlet line from the tubing connector. The seal wash reservoir line on the other side of the tubing connector can remain connected.

  6. Figure: Disconnecting the seal wash inlet line from the seal wash inlet port
  7. Connect the new seal wash line with a new tubing connector to the metering device head. Cut tubing to required length, if applicable.

  8. Guide the seal wash inlet line to the seal wash inlet port.

  9. Connect the seal wash inlet line to the tubing connector.

  10. Push the tubing connector with the lines into the seal wash inlet port.

Restarting the Seal Wash System

  1. Retighten the reservoir cap hand-tight. The retaining guide must remain in the hole in the reservoir cap. If it does not, press the retaining guide into the hole so that the tubing is kept in place in the cap.

  1. Check the wash lines over the entire flow path:

  1. Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
  2. If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
  1. Flush the seal wash system. Run the script: B01 - Change Liquids / Solvents (with: Rear Seal Wash Liquid and Refresh Only = Off.)