No peaks
Possible Cause | Remedial Action |
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Pre-heater used in nano or capillary LC applications | Remove the pre-heater (see Replacing the Passive Pre-Heater). |
Wrong capillaries used between column and detector or between autosampler and column | Use the correct capillaries for your application (to change the workflow and the flow connections, run the script: A06 - Change Fluidics / Workflow or refer to the Flow Scheme). |
Air bubbles in sample loop, needle, or metering device | Purge the autosampler. |
Vial empty | Check the sample level in the vial. If the sample level is too low, replace the vial with a filled one. |
Solvent in solvent reservoir does not match the solvent setting | Change the solvent in the solvent reservoir or change the solvent setting. |
Peak drift
Possible Cause | Remedial Action |
---|---|
Temperature of column compartment not stable |
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After changing the solvent type, the previous solvent has not been removed completely from the autosampler or system |
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