Pressure pulsation or inconstant pressure
Possible Cause | Remedial Action |
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System clogged with particles | Samples and insufficient solvent purity are frequently the source for particles clogging the system. In nano LC and capillary LC applications, the capillaries with even smaller inner diameters need an even higher level of solvent and sample purity.
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Solvent is degraded | Use fresh solvent. |
High backpressure
Possible Cause | Remedial Action |
---|---|
One or more capillaries in the system clogged or damaged by bending | Run the script: D01 - Test System Back Pressure. Follow the instructions. |
Column contaminated or clogged |
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Column switching valve clogged |
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Pre-heater clogged | Replace the pre-heater (see Replacing the Passive Pre-Heater). |
Contamination in the system | Flush the system by purging the pump and the autosampler with an appropriate solvent. |
Needle seat clogged | If the backpressure test script returns the message that there is a high backpressure in the needle seat, run the script: C21 – Clean Or Replace Needle Unit And Seat with the parameter: Clean Needle Seat. |
Solvent in solvent reservoir does not match the solvent setting | Change the solvent in the solvent reservoir or change the solvent setting. |
Low backpressure
Possible Cause | Remedial Action |
---|---|
Leak in system | Run the script: D02 - Test System Tightness (What Should Be Tested = System). Follow the instructions. |
Air trapped in the autosampler | Run the script: C04 - Purge Sampler. |
Solvent in solvent reservoir does not match the solvent setting | Change the solvent in the solvent reservoir or change the solvent setting. |