High baseline drift
Possible Cause | Remedial Action |
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System not sufficiently equilibrated |
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Unstable environmental conditions | Make sure that the temperature and the humidity are constant. |
Absorption of eluent changes when gradient is run | Absorbing additives may change the absorption spectrum, depending on the solvent. Consider varying additive concentrations to level the drift. |
Autosampler contaminated | Run the script: B03 - Clean Up System. |
Column contaminated | Clean or replace the column. |
Old solvent present in metering device head or sample loop | To purge the metering device or sample loop, run the script: C04 - Purge Sampler with the parameter Inner Needle Wash Strong Liquid. In general, when replacing the solvent reservoirs, run the script: B01 - Change Liquids / Solvents. |
Solvents degraded or inhomogeneous |
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Non-periodic baseline fluctuation, high noise
Possible Cause | Remedial Action |
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Air trapped in the system | Purge the pump and the autosampler. |
Solvent is degraded or of poor quality |
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Periodic baseline fluctuation, pulsation
Possible Cause | Remedial Action |
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Pressure fluctuations from pump |
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Air trapped in the system | Purge the pump. |