Follow the steps below to replace the peristaltic tubing in the seal wash pump.
When
Tubing is clogged or damaged
Every 6 months as preventive maintenance for the seal wash pump
Parts required
Peristaltic tubing (PharMedTM tubing), tubing connectors and holders
Tools required
Tubing cutter
Preparations
Empty the seal wash system:
- Unscrew the cap of the seal wash reservoir.
- Together with the cap, remove the seal wash line from the seal wash reservoir.
- To empty the seal wash line, lift up the lever of the seal wash pump. Release the lever when the line is empty.
Replacing the Peristaltic Tubing
No. | Description |
|---|---|
1 | Pump lever (lifted) |
2 | Pump rotor |
3 | Seal wash line from autosampler (silicone tubing) |
4 | Tubing connectors |
5 | Tubing holders for peristaltic tubing |
6 | Seal wash line to pump head (silicone tubing) |
7 | Peristaltic tubing |
Disconnect the peristaltic tubing from the tubing connectors that connect it to the silicone tubing.
Lift up the lever on the seal wash pump, and remove the peristaltic tubing from the pump and from the tubing holders.
Connect the new peristaltic tubing to the silicone tubing using the tubing connectors. Cut tubing to required length, if applicable.
Insert the peristaltic tubing into the top right tubing holder.
Lift up the lever on the seal wash pump and hold it in that position with one hand.
With your other hand, insert the tubing between the lever and the rotor and wind it around the rotor.
Release the lever.
Insert the peristaltic tubing into the bottom right tubing holder.
Insert the peristaltic tubing into the left tubing holder.
Check that the peristaltic tubing is inserted properly in the tubing holders. If it is not, push the tubing into the holders. Make sure that the tubing is not pinched or squeezed in the holders.
Restart the seal wash system.
Restarting the Seal Wash System
Retighten the reservoir cap hand-tight. The retaining guide must remain in the hole in the reservoir cap. If it does not, press the retaining guide into the hole so that the tubing is kept in place in the cap.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Flush the seal wash system. Run the script: B01 - Change Liquids / Solvents (with: Rear Seal Wash Liquid and Refresh Only = Off.)