CAUTION

Hot Columns

Columns inside the device may become hot during operation. Touching hot parts might cause burns.

Allow columns to cool down before starting replacement or maintenance procedures.

When

To change between columns

Parts required

Column as required by the application

Preparations

  1. Turn off the pump flow.

Follow these steps

  1. Remove the capillaries from the column inlet and column outlet.

  2. Remove the column that is to be replaced from the column clip. To remove the ring from the bottom part of the column clip, slightly press the ring in the direction of the arrows indicated on the ring and pull it towards you.

  3. Figure: Removing the ring
  4. Connect the capillaries to the inlet and outlet of the replacement column.

  5. Place the column into the bottom part of the column clip and reinstall the ring.

  6. Route the capillaries through one of the capillary guides shown in the figure.

  7. Figure: Capillary guides
  8. To set the column properties, run the scripts: A03 - Set Separation Column Type and A04 - Set Separation Column Specifications (and A05 - Set Trap Column Specifications, as required).

  9. Turn on the pump flow.