Before operating the system, fill the seal wash reservoir.
TIP
To avoid the formation of air bubbles in liquid lines, always place reservoirs in the solvent rack on top of the system stack.
Tools required
Tubing cutter
Preparations
Rinse the seal wash reservoir thoroughly. Use a high-purity solvent for rinsing.
Seal Wash Liquid
Use 75% isopropanol in water and 0.1% formic acid (HPLC-grade; preparation by volume, for example, 75 mL isopropanol + 25 mL water + 0.1 mL formic acid).
Follow these steps
No. | Description |
---|---|
1 | Wash line |
2 | Retaining guide |
3 | Reservoir cap |
4 | Cap plugs |
Feed the wash line through the retaining guide.
Feed the wash line through an open hole in the cap of the wash reservoir. The retaining guide keeps the tubing in place in the reservoir.
Close any open holes in the reservoir cap with cap plugs.
Fill the wash reservoir with wash liquid.
Tighten the reservoir cap hand-tight. Press the retaining guide into the hole in the reservoir cap so that the tubing is kept in place in the cap.
Place the wash reservoir in the solvent rack.
Check the wash lines over the entire flow path:
- Make sure that no bending (kink), pinching or squeezing of the wash lines is present at any point in the flow path.
- If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.