After installation of the sample loop, proceed as follows:

  1. Unlock the needle unit.

  2. Check that the sample loop moves freely when pushing the needle unit into the sample compartment.

    If the sample loop gets stuck on the interior panel, remove and reinstall the sample loop following the steps in this section. Make sure that the sample loop is aligned correctly and connector with capillary points to 6 o'clock.

  3. Mount the insulation cover.

  4. Turn on the autosampler with its main power switch. The autosampler performs a self-test. The needle initializes and adjusts its position.

  5. If required, set the Nominal Loop Volume parameter manually for the installed sample loop. Observe the volumes that are specified on the sample loop label.

Description

Nominal Loop
Volume

Total Loop
Volume

Sample Loop, 10 µL

10 µL

23 µL

Sample Loop, 25 µL

25 µL

50 µL

Sample Loop, 100 µL

100 µL

130 µL

  1. Prepare the autosampler for operation. See Preparing the System for Operation.