NOTICE

Particles and air bubbles can impair the system functionality

Some components of the system are filled with isopropanol when they were stored.

  • To flush out air bubbles (and isopropanol) before restarting the system or after installing additional components into the flow path, run the script: A02 - Auto Start Up.
  • To flush out isopropanol, use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent.

When

  • After turning on the system

  • After installing new components into the system flow path

Solvent and seal wash liquid volumes required

Needed liquid

Consumption during script execution: A02 - Auto Start Up

Consumption during analysis

Liquid requirements

Solvent

Max. 10 mL per solvent reservoir

Depends on the flow rate

If operating a component for the first time: Use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent.

In all other cases, see Solvent and Additive Information.

Seal wash liquid

Max. 10 mL

Approx. 250 mL per 14 days

See Filling the Seal Wash Reservoir.

Needle wash liquid volumes required

Consumption during the script: A02 - Auto Start Up

Consumption during analysis

Liquid requirements

Max. 10 mL

Consumption per needle wash reservoir and per injection1:

See Filling the Needle Wash Reservoirs.

SWP

500 µL

WWP

400 µL

W

250 µL

1: The values apply to: 25‑µL sample loop, direct injection (loop in bypass) set in chromatography data system, default wash settings of the chromatography data system.

Note: The consumption depends on the sample loop size, column size, wash time, workflow, and wash speed. If using other settings, the values can be significantly higher.

Follow these steps

  1. Check the liquid level of all reservoirs, especially that of the needle wash reservoirs. Make sure that the amounts are sufficient for the following:

    • Script A02 - Auto Start Up (see the table above)

    • First analysis (see the table above and add the amounts depending on the flow rate of your application).

  2. Close the doors of all modules in the Vanquish Neo system, if not already done.

  3. If you want to change the solvent type: : To set the solvent type of A/B, run the script: A01 - Set Pump Solvent Types.

  4. This step depends on the situation:

    • If you use the previously installed column: Check and, if necessary, adjust the configuration of the column using the scripts: A03 - Set Separation Column Type and A04 - Set Separation Column Specifications.

    • If you installed a new, previously unused, column: Configure the new column using the scripts: A03 - Set Separation Column Type and A04 - Set Separation Column Specifications.

  5. Run the script with or without diagnostics (leak test): A02 - Auto Start Up.

    The script flushes the system flow path up to the column inlet capillary, tests the system backpressure, and, optionally, performs a leak test.