NOTICE
Particles and air bubbles can impair the system functionality
Some components of the system are filled with isopropanol when they were stored.
When
After turning on the system
After installing new components into the system flow path
Solvent and seal wash liquid volumes required
Needed liquid | Consumption during script execution: A02 - Auto Start Up | Consumption during analysis | Liquid requirements |
---|---|---|---|
Solvent | Max. 10 mL per solvent reservoir | Depends on the flow rate | If operating a component for the first time: Use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent. In all other cases, see Solvent and Additive Information. |
Seal wash liquid | Max. 10 mL | Approx. 250 mL per 14 days |
Needle wash liquid volumes required
Consumption during the script: A02 - Auto Start Up | Consumption during analysis | Liquid requirements | |
---|---|---|---|
Max. 10 mL | Consumption per needle wash reservoir and per injection1: | ||
SWP | 500 µL | ||
WWP | 400 µL | ||
W | 250 µL | ||
1: The values apply to: 25‑µL sample loop, direct injection (loop in bypass) set in chromatography data system, default wash settings of the chromatography data system. Note: The consumption depends on the sample loop size, column size, wash time, workflow, and wash speed. If using other settings, the values can be significantly higher. |
Follow these steps
Check the liquid level of all reservoirs, especially that of the needle wash reservoirs. Make sure that the amounts are sufficient for the following:
Script A02 - Auto Start Up (see the table above)
First analysis (see the table above and add the amounts depending on the flow rate of your application).
Close the doors of all modules in the Vanquish Neo system, if not already done.
If you want to change the solvent type: : To set the solvent type of A/B, run the script: A01 - Set Pump Solvent Types.
This step depends on the situation:
If you use the previously installed column: Check and, if necessary, adjust the configuration of the column using the scripts: A03 - Set Separation Column Type and A04 - Set Separation Column Specifications.
If you installed a new, previously unused, column: Configure the new column using the scripts: A03 - Set Separation Column Type and A04 - Set Separation Column Specifications.
Run the script with or without diagnostics (leak test): A02 - Auto Start Up.
The script flushes the system flow path up to the column inlet capillary, tests the system backpressure, and, optionally, performs a leak test.