The figure shows the recommended fluidic setup for the use of two column switching valves.

Example: Setting up two column switching valves in the system fluidics, left: position 6-1, right: position 1-2
Figure: Example: Setting up two column switching valves in the system fluidics, left: position 6-1, right: position 1-2

Follow these steps

  1. Connect the Viper capillary from the pump or autosampler to port 3 of the bottom column switching valve.

  2. Depending on the column position, connect an active or passive pre-heater to port 2 or 4 of the bottom column switching valve and connect it to the column inlet.

  3. Connect a Viper capillary to port 1 or 5 of the top column switching valve and connect it to the column outlet.

  4. Connect a post-column cooler or a Viper capillary to port 6 of the top column switching valve and connect it to flow cell inlet of the detector.