This section describes how to connect and place the active pre-heater in the column compartment.

Parts required

  • Column holder

  • Active pre-heater

  • Viper inline filter (optional)

Preparations

NOTICE

The active pre-heater is filled with isopropanol when the column compartment is shipped from the manufacturing site. If of insufficient quality, isopropanol may damage the column.

  • Flush the components filled with isopropanol to waste, using solvents that are miscible with isopropanol. If they are not, use an appropriate solvent of similar polarity.
  • Install a Viper union in the flow path that replaces the column for the flushing procedure.

Follow these steps

TIP

Use a Viper inline filter between the active pre-heater and the column to prevent un-dissolved particles from getting into the column.

  1. Connect the Viper inline filter to the column.

  2. Connect the active pre-heater to the Viper inline filter.

  3. Attach the column holder to the active pre-heater fitting.

  4. Fix the column holder on the central wall inside the column chamber.

  5. Positioning pre-heater and column inside the column chamber
    Figure: Positioning pre-heater and column inside the column chamber
  6. Before you connect the pre-heater cable to the pre-heater connection port for the first time, check that the pre-heater is enabled in the Instrument Configuration Manager dialog box for the column compartment.

    Once the pre-heater is enabled in the chromatography data system, it detects automatically when the pre-heater is connected.

  7. Connect the pre-heater cable to the pre-heater connection port.

    The pre-heater connection ports are located next to the lower column switching valve.

  8. TIP

    Use the left connection port for a pre-heater installed in the left column slot, and the right connection port for a pre-heater installed in the right column slot.

  9. Pre-heater connection port
    Figure: Pre-heater connection port
  10. Route the pre-heater cable through the pre-heater cable guide. Each pre-heater cable and cable guide is located above the pre-heater port. If the pre-heater cable is too long, loop the cable in the guide.

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Figure: Pre-heater cable and cable guide; on the right: cable looped in guide

No.

Description

1

Cable guide

2

Pre-heater cable