Inside the column chamber, a central wall separates the left column position from the right one. Use a column holder to fix the columns on the central wall inside the column chamber.

Column holders
Figure: Column holders

No.

Description

1

Column holder for left-side positioning of the column

2

Column holder for placing two columns at the same height

3

Column holder for right-side positioning of the column

Follow these steps

  1. Connect the capillaries to the column inlet and column outlet.

  2. NOTICE

    To avoid any leakage, check that all fittings are properly connected to the column inlet and outlet, when installing or exchanging capillaries.

  3. Attach the column holder to the column fitting.

  4. Attaching column holder to column fitting
    Figure: Attaching column holder to column fitting
  5. Fix the column holder on the central wall inside the column chamber.

    The column holder can be fixed at any position on the central wall.

  6. Positioning the column inside the column chamber
    Figure: Positioning the column inside the column chamber
  7. NOTICE

    To avoid any damage to the central wall, do not slide the holders along the wall. If you need to change their position, pull off the holder and fix it again at the desired position.

  8. Route the capillaries through the capillary guides. The capillary guides are located above and below the column chamber.

Figure: Routing the capillary through the capillary guide