To reduce the temperature of the liquid leaving the column, install a post-column cooler. Post-column cooling minimizes the detector noise and optimizes the detection results.
NOTICE
With Vanquish systems including a detector with LightPipe™ flow cell, always use post-column cooling to reduce the temperature of the liquid leaving the column to values below 50 °C. Higher temperatures will damage the flow cell.
Parts required
Post-column cooler
Follow these steps
Open the column compartment door.
Attach the post-column cooler to the mounting plate above the column chamber.
The post-column cooler can be installed either side, depending on the location of the detector:
If the detector is located left of the column compartment, the capillary fixed on the side of the post-column cooler should point to the left.
If the detector is located right of the column compartment, the capillary of the post-column cooler should point to the right.
You can turn the post-column cooler upside down: If the hot column eluate comes from the upper column switching valve, the capillary should point upwards. If the hot eluate comes from the column, the capillary should point downwards.
To fix the post-column cooler to the mounting plate, tighten the 2 thumb screws.
Configure the post-column cooler in the chromatography data system:
In the Instrument Configuration Manager dialog box for the column compartment, enable the post-column cooler.
TIP
When removing the post-column cooler, disable the post-column cooler in the Instrument Configuration Manager dialog box for the column compartment.