This section gives information on any additional steps that are required to prepare the device for operation and sample analysis.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for the first time:
Before Starting Sample Analysis
Before starting an analysis:
Check the liquid level in the solvent reservoirs. Verify that the amount of solvent is sufficient for the analysis.
Close the doors of all modules in the Vanquish system, if not already done.
Make sure that the chromatography system is properly equilibrated (see further down).
System Equilibration
System equilibration should include the following operations:
Purging the pump (all channels, including those not used for the application)
Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run
Warming up (or cooling down) all temperature-controlled devices in the system to the starting temperature. Temperature-controlled devices can be, for example
Column compartment and post-column cooler
Sample compartment thermostatting in the autosampler
Flow cell in a fluorescence detector
Evaporation tube in a charged aerosol detector
Turning on the lamp (or lamps) in the UV/VIS detector
Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application
Monitoring the detector signal and checking whether the detector signal is stable so that the drift and signal noise are within reasonable limits for the application
Performing an autozero of the detector baseline
TIP
The Chromeleon software supports procedures for automatically starting a chromatography system in the software (Smart Startup). The startup procedure includes the operations for system equilibration. For details, refer to the Chromeleon Help.