To interrupt operation for a longer period, follow the instructions below.
TIP
Shutting down the device affects the operation of the system. When shutting down the device, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).
Remove the column.
Flush the system with an appropriate solvent (minimum HPLC-grade). Observe the following:
TIP
With a Vanquish Core system that has been modified for using normal-phase compatible solvents and additives, refer to the information about the flushing liquid in the Considerations with Normal-Phase Compatible Solvents and Additives section in the Vanquish System Operating Manual.
Situation after Shutdown | If no additive is used | If an additive is used |
|---|---|---|
Device remains in the laboratory after shutdown | Flush the system, for example with methanol. 100% acetonitrile should not be used. | Flush the system with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent. |
Device shall be transported or shipped after shutdown | Flush the system with isopropanol. | Flush the system first with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent. Afterward, flush the system with isopropanol. |
Turn off the pump flow. Wait until the system pressure is down to zero before you continue the shutdown of the device.
The step depends as follows:
Situation | Steps |
|---|---|
Device and all other system modules remain in the system stack and are to be turned off | Turn off the system with the system power button on the system base. |
Device shall be transported or shipped after shutdown | If one of the modules shall be removed from the system stack, turn off all system modules with their main power switch. Pressing the system power button will not be sufficient to turn off the power to the devices completely. Follow the instructions in Transporting or Shipping the Device. |