• The pump should deliver continuous flow and at the same time ensure the intermixing of solvents from different reservoirs, if applicable.

  • The mobile phase reservoirs should be clean borosilicate glass that is dedicated for use with volatile mobile phases.

    In some applications, however, it may be necessary to use plastic reservoirs, for example when using extreme pH aqueous eluents (such as > pH 12).

  • Consider the pump flow rate limit of the detector when preparing analysis. Operate the detector only within the allowed pump flow rate range.

  • It is recommended to use online degassers for HPLC systems with the detector. Make sure to thoroughly flush any degasser channel that has been exposed to non-volatile buffers. In some cases, it may be necessary to dedicate degasser channels to only use volatile mobile phases.