To interrupt operation for a longer period, follow the instructions below.

NOTICE

Observe the following to avoid damage to the charged aerosol detector:

  • Always flush the detector with an appropriate pure solvent before interrupting operation. Follow the steps below.
  • Avoid leaving the detector exposed to highly acidic or basic mobile phases for long periods.
  • If the detector was operated with organic solvents in high concentration that may form peroxides upon evaporation, such as un-stabilized tetrahydrofuran or isopropanol, flush the detector with LC/MS-grade water before shutdown.

TIP

Shutting down the device affects the operation of the system. When shutting down the device, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).

  1. Remove the column.

  2. Flush the system with an appropriate solvent (minimum HPLC-grade). Observe the following:

Situation after Shutdown

If no additive is used

If an additive is used

Device remains in the laboratory after shutdown

Flush the system, for example with methanol. 100% acetonitrile should not be used.

Flush the system with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent.

Device shall be transported or shipped after shutdown

Flush the system with isopropanol.

Flush the system first with several volumes of methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent. Afterward, flush the system with isopropanol.

    Make sure that residual sample components, impurities from the column or buffers are completely removed from the detector.

    TIP

    As an intermediate solvent, you can use 100% methanol to flush the charged aerosol detector.

    1. Turn off the pump flow and then the gas flow:

    1. Turn off the pump flow to the detector.
    2. Wait at least 30 minutes. During the waiting period, the detector drains any remaining mobile phase to waste.
    3. NOTICE

      Damage to the detector

      Turning off the gas glow too early may result in condensation and, thus, to deterioration of detector parts and/or flooding.

      Wait for at least 30 minutes before you turn off the gas flow to the detector.

    4. Turn off the gas flow on the detector.
    5. Wait until the system pressure is down to zero before you continue.
    6. Turn off the gas supply to the detector.
    1. The step depends as follows:

    Situation

    Steps

    Device and all other system modules remain in the system stack and are to be turned off

    Turn off the system with the system power button on the system base.

    Device shall be transported or shipped after shutdown

    If one of the modules shall be removed from the system stack, turn off all system modules with their main power switch. Pressing the system power button will not be sufficient to turn off the power to the devices completely.

    Follow the instructions in Transporting or Shipping the Device.