The Identification subpage of the processing method editing wizard contains up to four editable sections: Sequence Matching Mass Tolerance, Multiconsensus Component Merge, Sequences Added to Experiment (table), and Global Sequence Reference (table).
NOTE
The Sequence Matching Mass Tolerance and Multiconsensus Component Merge areas are active only if you select the Intact Deconvolution checkbox for at least one of the peaks added on the Component Detection subpage of the processing method editing wizard.
These sections are inactive for experiments containing MS/MS data or experiments containing full-scan MS data with the Intact Deconvolution option disabled.
As needed, you canadd, delete, or edit sequences for the experiment using the Sequences Added to Experiment and Global Sequence Reference tables.
| No. | Description | No. | Description |
|---|---|---|---|
1 | Parameters tab (processing method editing wizard) | 4 | Multiconsensus Component Merge parameters (for Intact Deconvolution) |
2 | Subpage navigation bar | 5 | Sequences Added to Experiment table |
3 | Sequence Matching Mass Tolerance (for Intact Deconvolution) | 6 | Global Sequence Reference table |
The table below describes the parameters on the Identification subpage.
Parameter | Description |
|---|---|
Sequence Matching Mass Tolerance | Specifies the mass tolerance (in ppm or Da) within which the mass of the full protein sequence and individual components must fall to be considered a match. |
Multiconsensus Component Merge | |
Deconvolution Mass Tolerance | Specifies the mass tolerance (in ppm or Da) that components from individual raw data files must meet to be merged into a multiconsensus component. The application excludes individual raw data file components whose masses differ by more than this tolerance from a multiconsensus component. |
RT Tolerance | Specifies the maximum difference in retention time (RT) that components from individual files must meet to be merged into a multiconsensus component. The application excludes individual raw data file components whose retention times are separated by more than this gap from a multiconsensus component. |
Minimum Number of Required Occurrences | Specifies the minimum number of individual raw data files that must be included in a multiconsensus component for it to be considered valid. The application excludes multiconsensus components comprised of fewer than this number of files. |