The Component Detection subpage of the processing method editing wizard displays the editable parameters related to component detection in your Top Down Analysis experiment. These parameters vary, depending on the raw data files and deconvolution algorithm (Xtract or ReSpect) that you select for the experiment.
IMPORTANT
In the Top Down Analysis workflow, it is important to apply the correct data processing method (Intact Deconvolution or Intact Fragmentation) and deconvolution algorithm (Xtract or ReSpect) depending on the type of MS data acquired for the experiment.
- In the Peak # - Deconvolution Parameters pane, choose either of the following data processing methods:
- Intact Deconvolution: For full-scan MS data
- Intact Fragmentation: For MS/MS data does not respect
- Then, choose either of the following deconvolution algorithm:
- ReSpect: For isotopically-unresolved MS data
- Xtract: For isotopically-resolved MS data (available for Intact Fragmentation only)
Refer to the following tables for more information about the parameters in each area on the Component Detection page.
Select the Show Advanced Parameters checkbox to edit advanced options that are hidden by default. Normally, these advanced parameters do not need to be edited.
On the right side of the Component Detection page, the Chromatogram, Source Spectrum panes display plots for each raw data file in the experiment, with a separate tab for each raw data file.
| No. | Description | No. | Description |
|---|---|---|---|
1 | Parameters tab (processing method editing wizard) | 8 | Intact Fragmentation and Intact Deconvolution tabs |
2 | Subpage navigation bar | 9 | Deconvolution Algorithm selection |
3 | Peak Selection pane | 10 | Show Advanced Parameters checkbox |
4 | Peak Selection parameters | 11 | Chromatgram |
5 | Add Peak button | 12 | Intact Fragmentation Source Spectrum pane |
6 | Intact Deconvolution (Optional) checkbox | 13 | Intact Deconvolution Source Spectrum pane |
7 | Peak # - Deconvolution Parameters pane | 14 | Raw data file tabs |