You can use the data acquisition software to automatically inject samples from an autosampler. In a typical LC/MS experiment, solvent flow goes through an LC column to separate the compounds of a mixture before directing them into the API source, either H-ESI or APCI.

To set up injections from an LC system with an autosampler

Procedure

  1. Use red PEEK tubing and a Rheodyne fitting to connect port 5 of the divert/inject valve to one of the following:
  2. The outlet of an autosampler (not shown)
  3. The outlet of a liquid chromatography column that is attached to an autosampler (not shown)
  4. Use a Rheodyne fitting to connect one end of the Teflon or PEEK tubing to port 4. Place the other end of the tubing in a waste container (not shown).
  5. Connect red PEEK tubing to port 6 and the other end to the API source.
  6. Divert/Inject valve plumbed as a divert valve
    123
    No.DescriptionNo.Description

    1

    Port 6 flow goes to the API source

    3

    Teflon or PEEK tubing connects port 4 to waste

    2

    Port 5 flow comes in from the LC column or autosampler