The high-flow infusion method uses an LC Tee union to direct the solvent flow from the syringe pump into the solvent flow produced by an LC pump. Use this infusion method to perform experiments at a higher flow rate with an LC system. The high-flow infusion method lets you optimize the source parameters (such as sheath gas and vaporizer temperature) at the flow rate and mobile phase composition of the instrument method.
Before you begin, complete the procedures in Syringe and syringe pump setup.
After you set up the Tee union, connect the API source.
- For H-ESI or low-flow H-ESI, see H-ESI or H-ESI low-flow direct infusion.
- For APCI, see Connect the sample flow to the API source.
To set up the system for high-flow infusion without an autosampler
Procedure
- Fill the syringe with your sample and connect it to the syringe pump (not shown).
- Using red PEEK tubing and natural PEEK fittings, connect the syringe union to the base of the Tee union.
- This is the infusion line.
- Use a fingertight fitting to connect a piece of red PEEK tubing to one side of the Tee union.
- This connection adds the flow from the LC pump to the flow from the syringe pump.
- Using red PEEK tubing and fittings, connect the open end of the Tee union to the grounding of the union source.
- This brings the flow to the MS.
High-flow infusion without an autosampler No. Description No. Description 1
Syringe
4
Red PEEK tubing connecting the Tee valve to the MS
2
Syringe adapter and union
5
Tee union
3
Red PEEK tubing infusion line
6
PEEK tubing from the LC system