You can configure (plumb) the divert/inject valve as a loop injector (for flow injection analysis) or as a divert valve. The divert valve can switch the solvent front, gradient endpoint, or any portion of the LC run to waste. For details, see these topics:

Loop injection

In the loop injection valve configuration, the valve switches between these two positions:

  • Load (position 1–2)—The sample loop is isolated from the solvent stream. Solvent flow from the LC pump enters and exits the valve through ports 5 and 6, respectively. When you load the sample into port 2, the sample enters and exits the sample loop through ports 1 and 4, respectively. As you overfill the sample loop, the excess sample exits the valve through port 3 to waste.
  • Inject (position 1–6)—The sample loop is open to the solvent stream. The solvent flow from the LC pump flushes sample out of the sample loop, and then exits through port 6 to the sample transfer line.
Loop injection positions
12
No.DescriptionNo.Description

1

Load (position 1–2)

2

Inject (position 1–6)

Divert valve

In the divert valve configuration, the valve switches between these two positions:

  • Detector (position 1–2)—Solvent flow from the LC pump enters the valve through port 5 and exits through port 6 to the sample transfer line.
  • Waste (position 1–6)—Solvent flow from the LC pump enters the valve through port 5 and exits through port 4 to waste
Divert/Inject valve plumbed as a divert valve
123
No.DescriptionNo.Description

1

Port 1 to API source

3

Port 3 to waste

2

Port 2 from LC pump

When the solvent flow is connected to a UV detector, use the setup shown in Set up injections using the autosampler.