Procedure

  1. Using a polymeric tubing cutter, cut the ends of a 0.6 m (2 ft) length of red PEEK tubing so that they are square.
  2. IMPORTANT

    Poorly cut tubing can cause flow restrictions.

  3. Make sure that the PEEK tubing is not crimped, kinked, or otherwise damaged.
  4. Connect the PEEK tubing so that it contacts the bottom of the LC union’s 10-32, coned-bottom receiving port.
  5. IMPORTANT

    Tubing that is not properly seated can add dead volume to a chromatographic system.

  6. Tighten the PEEK fittings using only your fingers. Overtightening the PEEK fittings can cause leaks.
  7. Syringe connection to the sample transfer line
    12
    No.DescriptionNo.Description

    1

    Syringe union

    2

    Sample transfer line