Use the syringe pump to infuse a sample into the solvent stream that is produced by an LC system or to load a sample into the divert/inject valve.

IMPORTANT

To minimize the possibility of cross-contamination, do the following:

  • Use a dedicated syringe and length of PEEK tubing for the calibration solution and another syringe and length of PEEK tubing for sample solutions.
  • Wipe off the needle tip with a clean, lint-free tissue before reinserting the syringe into the syringe union adapter assembly.

Procedure

  1. Load a clean 500 μL syringe with the sample solution.
  2. Use a fingertight fitting to connect a 4 cm (1.5 in.) length of Teflon tubing to the (black) syringe union adapter.

  3. CAUTION

    Sharp object

    The syringe needle can puncture your skin. Handle it with care.

  4. Hold the plunger of the syringe in place and carefully insert the tip of the syringe needle into the free end of the Teflon tubing. Then, place the syringe on the syringe pump.
  5. Syringe needle inserted into the Teflon tubing
    Syringe needle inserted into the Teflon tubing

  6. TIP

    If necessary, use the syringe needle tip to slightly enlarge the opening in the end of the Teflon tubing.

  7. Squeeze the syringe pump’s release button and slowly move the pusher block until it contacts the flange on the end of the syringe plunger.
  8. Syringe pump with syringe
    123
    No.DescriptionNo.Description

    1

    Syringe pump release button on the pusher bar

    3

    Syringe needle and Teflon tubing connection to the union

    2

    Syringe with sample solution