Optimize the source parameters in the optimization pane - TSQ Quantis Plus MS - TSQ Fortis Plus MS - TSQ Series - TSQ Altis MS - TSQ Quantis MS - TSQ Altis Plus MS - TSQ Fortis MS - Optimize the source parameters in the optimization pane - TSQ Quantis Plus MS - TSQ Fortis Plus MS - TSQ Series - TSQ Altis MS - TSQ Altis MS - TSQ Quantis MS - TSQ Quantis MS - TSQ Altis Plus MS - TSQ Fortis MS

Procedure

1. Make sure that the syringe has enough of the sample solution.
2. Plumb the system for high-flow infusion. See Set up the MS system for compound optimization.
3. Place the system in On mode.
4. In the Tune window, select the Ion Source tab to display the Ion Source pane. Then, enter the source conditions provided by the Set Default feature for the flow rate that you are using.
5. Set the mobile phase from the LC system to the flow rate and solvent composition in the instrument method.
6. In the Tune window, select Syringe Off to turn on the syringe pump.
7. The button name changes to Syringe On.
7. Select the Optimization tab. Then, do the following in the Optimization pane:
1. Under Injection Settings, select Syringe in the Sample Injection Type list.

2. 

2. In the Mass List Type list, select Precursor or Formula.
1. For the Formula mass list type, select one or both ion polarity modes from the Polarity list. Then, specify the adducts to include.
2. For the Precursor mass list type, select the Positive polarity mode or the Negative polarity mode.

3. IMPORTANT

   Thermo Fisher Scientific recommends using the theoretical masses for the precursor masses. Enter that mass into the table.

3. In the Source Optimization list, select On and then select the Spray Voltage check boxes..
4. For a SIM scan, select the Source Fragmentation check box and, depending on MS, the RF Lens check box or the Tube Lens check box. For an SRM scan, also select On in the Product Ion Optimization list.

5. NOTE

   Other than the number of product ions, the default values are sufficient for most experiments:

   • Product Ion Type: Unknown
   • Number of Product Ions: 1 (adjust this setting as needed)
   • Exclude Loss Mass (m/z): None
   • Low Mass Exclusion (m/z): 10
5. Select the Add/Remove Table Column icon next to the parameters that you want to customize per compound. Otherwise, the MS applies the parameter setting to all compounds.
6. 
   
7. When selected, the icon changes color from to , and the parameter appears as an additional column in the Optimization Table.

8. 

8. In the Optimization Table, add a row for each compound and modify the column values as applicable.

9. TIP

   If there are multiple syringes, enter the syringe number in the Injection ID column.

10. 

9. Select Start.