The calibration parameters affect mass accuracy and resolution.

The tune parameters affect the magnitude of the ion signal. There are two types of tune parameters:

  • Mass-dependent parameters: DC voltages of L0 lens, Turner-Kruger Lens 1 (TK1), and the DC offset voltages of the mass-analyzing quadrupoles and their end segments.
  • Compound-dependent parameters: spray voltage (H-ESI or N-ESI), spray current (APCI), sheath gas pressure, auxiliary gas pressure, sweep gas pressure, vaporizer temperature, and ion transfer tube temperature. The instrument control software does not optimize the compound-dependent parameters during an EMRS calibration. For information about optimizing the signal for your analytes, see Optimize the compound signal.

NOTE

When the MS completes a calibration, the Tune application writes the calibration parameters and the mass-dependent tune parameters to the calibration file, which overwrites the existing file. You cannot replace or modify the calibration file.

The Tune application writes the compound-dependent tune parameters to a change record in the History pane. You can rename and save the change record to the Favorites pane for future use in the Tune application or the Method Editor.