Successful calibration of the MS requires a steady and stable flow rate of the EMRS.
The System Calibration routine performs the following steps:
- Checks the spray stability. If the spray stability check passes, the routine performs the remaining tuning and calibration steps.
- Calibrates the EM Gain.
- Tunes Q1 and Q3.
- Calibrates the mass analyzers by using the mass position and resolution of the peaks in the EMRS spectrum.
IMPORTANT
Do not operate the system if it fails the calibration routine, as the quality of your data is based on the mass accuracy and resolution of the MS. Contact Thermo Fisher Scientific Technical Support for assistance.
IMPORTANT
- For optimum performance, calibrate the MS every one to three months of operation.
- To reduce the risk of potential errors, monitor quality control standards frequently.
Prerequisites
- Prepare the MS for calibration. See Prepare the MS system for calibration.
Procedure
- Select the Calibration tab.
- In the Calibration pane, select the System Calibration option.
Calibration pane, showing the selection of the System Calibration option - Select Start.
- The Stop button replaces the Start button and the System Calibration routine begins with a Spray Stability check.
Beginning of the System Calibration routine - When the calibration is complete, the Tune application prompts you to generate a report of the calibration results.
Report Generation Options - At the prompt, choose an option and select OK.
- After the MS completes the calibration procedure in positive mode, run the calibration routine in negative ion mode.