After you start the flow of the calibration solution to the ion source, monitor the full scan spectrum of the calibration solution.
Procedure
- If you have not already applied the favorites setting for the polarity mode you are calibrating, apply it now.
- The favorites setting for the positive polarity mode sets the scan mode to Full Scan (Q1) with a mass range from 50 to 1650 m/z.
- Inspect the spectrum in the spectrum view of the Tune window.
- For the positive mode, check the peaks at the following m/z values:
69, 102, 215, 622, 922, and 1522. - For the negative mode, check the peaks at the following m/z values:
69, 113, 302, 602, 1034, and 1634. - For each of the expected peaks, check that the peak is the most abundant peak within ±10 amu of the expected m/z value.
- If necessary, adjust the gas settings in the Ion Source pane, especially the Aux Gas parameter setting or the Sweep Gas parameter setting.
- Check for the following interfering or missing m/z values:
- For the positive mode, check for contaminating peaks at m/z 100 and m/z 212. Possible sources of the m/z 100 contaminant include the nitrogen line or the ion source housing. The contaminant at m/z 212 is probably caused by wearing nitrile gloves while working with the EMRS.
- For the negative mode, check for a missing or small peak at m/z 302. The peak at m/z 302 disappears as the EMRS degrades. If the peak is too small or missing, fill the syringe with fresh solution.