The Tune application includes favorites settings for calibrating the MS with EMRS.
Procedure
- In the Tune window, place the MS in Standby mode
. NOTE
Placing the MS in Standby mode prevents the ion transfer tube from heating to a temperature setting that is too high for the calibration procedure.
- In the data type list, and select the Profile data type.
- Select the Define Scan tab.
- In the Define Scan pane, select the Full Scan mode.
- Under System Settings in the Favorites pane, do one of the following:
- To calibrate the MS in the positive ion mode with the calibration probe, right-click ESI Default for EMRS Calibration with Positive Polarity and select Apply.
- To calibrate the MS in the negative ion mode with the calibration probe, right-click ESI Default for EMRS Calibration with Negative Polarity and select Apply.
- To calibrate the MS in the positive ion mode with the H-ESI probe, right-click H-ESI Default for EMRS Calibration with Positive Polarity and select Apply.
- To calibrate the MS in the negative ion mode with the H-ESI probe, right-click H-ESI Default for EMRS Calibration with Negative Polarity and select Apply.
System Settings menu with the available favorites for the calibration probe Default parameter settings for the calibration with the EMRS in the positive polarity mode for the H-ESI probe
Parameter | Positive polarity mode | Negative polarity mode |
|---|---|---|
Spray Voltage (V) | 3500 | 3200 |
Sheath Gas (Arb) | 0 | 0 |
Aux Gas (Arb) | 0 | 0 |
Sweep Gas (Arb) | 0 | 0 |
Ion Transfer Tube Temp (°C) | 325 | 325 |
Vaporizer Temperature (°C) | Parameter not available with ESI | Parameter not available with ESI |
Source Fragmentation (V) | 30 (20 for TSQ Fortis) | 30 (For all) |
Syringe pump flow rate (μL/min) | 2 to 5 | 2 to 5 |
- In the Tune window, select the Ion Source tab. Then, check the settings in the Ion Source pane.
Default ion source settings for the H-ESI probe